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CALR elisa kit :: Human calreticulin ELISA Kit

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Catalog # MBS703504
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $550 +1 FREE 8GB USB
  96-Strip-Wells  /  $795 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

calreticulin, ELISA Kit

Popular Item
Also Known As

Human Calreticulin, CRT ELISA Kit

Product Synonym Names
Human Calreticulin; CRT ELISA Kit; CRT; FLJ26680; RO; SSA; cC1qR; Sicca syndrome antigen A (autoantigen Ro; calreticulin) ; autoantigen Ro; calreticulin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS703504 Testing Data
Sequence Length
417
OMIM
109091
3D Structure
ModBase 3D Structure for P27797
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human CRT. No significant cross-reactivity or interference between human CRT and analogues was observed.
Samples
Serum, plasma, tissue homogenates, urine.
Assay Type
Sandwich
Detection Range
0.156 ng/ml -10 ng/ml.
Sensitivity
0.039 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CALR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCALRpurchase
MBS703504 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the calreticulin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CALR. The ELISA analytical biochemical technique of the MBS703504 kit is based on CALR antibody-CALR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CALR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CALR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CALR elisa kit
Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CALR elisa kit
CALR elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS703504. Click to request current manual
NCBI/Uniprot data below describe general gene information for CALR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
48,142 Da
NCBI Official Full Name
calreticulin
NCBI Official Synonym Full Names
calreticulin
NCBI Official Symbol
NCBI Official Synonym Symbols
RO; CRT; SSA; cC1qR; HEL-S-99n
  [Similar Products]
NCBI Protein Information
calreticulin; CRP55; ERp60; HACBP; grp60; calregulin; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; Sicca syndrome antigen A (autoantigen Ro; calreticulin)
UniProt Protein Name
Calreticulin
UniProt Synonym Protein Names
CRP55; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP; grp60
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CRTC; ERp60  [Similar Products]
UniProt Entry Name
CALR_HUMAN
NCBI Summary for CALR
Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq, Jul 2008]
UniProt Comments for CALR
Function: Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis 2 PublicationsManual assertion based on experiment in:Ref.20
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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