• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CAMKK2 elisa kit :: Human Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2000155 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  24-Strip-Wells  /  $285 +1 FREE 8GB USB
  48-Strip-Wells  /  $535 +1 FREE 8GB USB
  96-Strip-Wells  /  $725 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,945 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,345 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2), ELISA Kit

Full Product Name

Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) ELISA Kit

Product Synonym Names
CAMKK; CAMKKB; Calcium/calmodulin-dependent protein kinase kinase beta
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
114180
3D Structure
ModBase 3D Structure for P62158
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2).
No significant cross-reactivity or interference between Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates and Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.114ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CAMKK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CAMKK2purchase
MBS2000155 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CAMKK2. The ELISA analytical biochemical technique of the MBS2000155 kit is based on CAMKK2 antibody-CAMKK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CAMKK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CAMKK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CAMKK2 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for CAMKK2 elisa kit

Typical Testing Data/Standard Curve (for reference only) of CAMKK2 elisa kit
CAMKK2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2000155. Click to request current manual
NCBI/Uniprot data below describe general gene information for CAMKK2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,838 Da
NCBI Official Full Name
calmodulin
NCBI Official Synonym Full Names
calmodulin 1 (phosphorylase kinase, delta)
NCBI Official Symbol
CALM1  [Similar Products]
NCBI Official Synonym Symbols
caM; CAMI; PHKD; CPVT4; DD132; LQT14; CALML2
  [Similar Products]
NCBI Protein Information
calmodulin
UniProt Protein Name
Calmodulin
UniProt Gene Name
CALM1  [Similar Products]
UniProt Synonym Gene Names
CALM; CAM; CAM1; CaM  [Similar Products]
UniProt Entry Name
CALM_HUMAN
NCBI Summary for CAMKK2
This gene encodes a member of the EF-hand calcium-binding protein family. It is one of three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. Two pseudogenes have been identified on chromosome 7 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
UniProt Comments for CAMKK2
Calmodulin: a calcium-binding small regulatory protein that mediates the control of a large number of enzymes by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Has four functional calcium-binding domains. Phosphorylation and ubiquitination result in a decreased activity.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 14q32.11

Cellular Component: nucleoplasm; spindle pole; centrosome; sarcomere; growth cone; spindle microtubule; cytoplasm; plasma membrane; extracellular region; nucleus; cytosol; vesicle

Molecular Function: protein domain specific binding; nitric-oxide synthase regulator activity; titin binding; calcium ion binding; calcium-dependent protein binding; protein kinase binding; protein serine/threonine kinase activator activity; protein binding; N-terminal myristoylation domain binding; phospholipase binding; thioesterase binding; type 3 metabotropic glutamate receptor binding; phosphoinositide 3-kinase binding; nitric-oxide synthase binding; adenylate cyclase binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; phototransduction, visible light; inositol phosphate metabolic process; nerve growth factor receptor signaling pathway; pathogenesis; signal transduction; nitric oxide metabolic process; positive regulation of protein amino acid dephosphorylation; synaptic transmission; platelet degranulation; substantia nigra development; muscle contraction; positive regulation of cyclic nucleotide metabolic process; response to corticosterone stimulus; epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; platelet activation; glycogen catabolic process; fibroblast growth factor receptor signaling pathway; regulation of heart rate; positive regulation of nitric-oxide synthase activity; organelle organization and biogenesis; adenylate cyclase activation; response to amphetamine; glucose metabolic process; regulation of cytokinesis; positive regulation of protein amino acid autophosphorylation; rhodopsin mediated signaling; G-protein coupled receptor protein signaling pathway; regulation of rhodopsin mediated signaling; phospholipase C activation; carbohydrate metabolic process; innate immune response; positive regulation of cyclic-nucleotide phosphodiesterase activity; regulation of nitric-oxide synthase activity; detection of calcium ion; vascular endothelial growth factor receptor signaling pathway; blood coagulation; response to calcium ion

Disease: Long Qt Syndrome 14; Ventricular Tachycardia, Catecholaminergic Polymorphic, 4
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000