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Camp elisa kit :: Mouse cyclic Adenosine Monophosphate ELISA Kit

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Catalog # MBS722602
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

cyclic Adenosine Monophosphate (Camp), ELISA Kit

Popular Item
Also Known As

Mouse cyclic Adenosine Monophosphate ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
600474
3D Structure
ModBase 3D Structure for P49913
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of cAMP. No significant cross-reactivity or interference between cAMP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between cAMP and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
10-250pmoL/mL
Sensitivity
1.0pmoL/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Camp elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCamppurchase
MBS722602 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cyclic Adenosine Monophosphate (Camp) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Camp. The ELISA analytical biochemical technique of the MBS722602 kit is based on Camp antibody-Camp antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Camp antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Camp. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Camp elisa kit
Intended Uses: This cAMP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse cAMP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||CAMP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for cAMP. Standards or samples are then added to the microtiter plate wells and cAMP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of cAMP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for cAMP are added to each well to "sandwich" the cAMP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain cAMP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cAMP concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Camp elisa kit

Typical Testing Data/Standard Curve of Camp elisa kit
Camp elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS722602. Click to request current manual
NCBI/Uniprot data below describe general gene information for Camp. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
19,301 Da
NCBI Official Full Name
CAMP, partial
UniProt Protein Name
Cathelicidin antimicrobial peptide
UniProt Synonym Protein Names
18 kDa cationic antimicrobial protein; CAP-18; hCAP-18
UniProt Gene Name
UniProt Synonym Gene Names
CAP18; FALL39; HSD26; CAP-18; hCAP-18  [Similar Products]
UniProt Entry Name
CAMP_HUMAN
NCBI Summary for Camp
This gene encodes a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. [provided by RefSeq, Sep 2014]
UniProt Comments for Camp
CAMP: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. Belongs to the cathelicidin family.

Protein type: Secreted, signal peptide; Secreted; Cell cycle regulation; Vesicle

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: extracellular space; specific granule; cell projection; cell wall; cytosol

Molecular Function: protein binding

Biological Process: interaction with host; defense response to Gram-positive bacterium; positive regulation of angiogenesis; innate immune response in mucosa; killing by host of symbiont cells; defense response to bacterium; positive regulation of cell proliferation; antibacterial humoral response; positive regulation of protein amino acid phosphorylation; defense response to Gram-negative bacterium
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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