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CAPNS2 elisa kit :: Rabbit Calpain small subunit 2 (CAPNS2) ELISA Kit

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Catalog # MBS7222379
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

Calpain small subunit 2 (CAPNS2), ELISA Kit

Popular Item
Also Known As

Rabbit Calpain small subunit 2 (CAPNS2) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of CAPNS2. No significant cross-reactivity or interference between CAPNS2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CAPNS2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CAPNS2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCAPNS2purchase
MBS7222379 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calpain small subunit 2 (CAPNS2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CAPNS2. The ELISA analytical biochemical technique of the MBS7222379 kit is based on CAPNS2 antibody-CAPNS2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CAPNS2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CAPNS2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CAPNS2 elisa kit
Intended Uses: This CAPNS2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit CAPNS2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||CAPNS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CAPNS2 antibody and an CAPNS2-HRP conjugate. The assay sample and buffer are incubated together with CAPNS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAPNS2 concentration since CAPNS2 from samples and CAPNS2-HRP conjugate compete for the anti-CAPNS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAPNS2 from the sample, fewer sites are left to bind CAPNS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAPNS2 concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve of CAPNS2 elisa kit
CAPNS2 elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS7222379. Click to request current manual
NCBI/Uniprot data below describe general gene information for CAPNS2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,660 Da
NCBI Official Full Name
calpain small subunit 2
NCBI Official Synonym Full Names
calpain, small subunit 2
NCBI Official Symbol
CAPNS2  [Similar Products]
NCBI Protein Information
calpain small subunit 2; CSS2; calcium-dependent protease small subunit 2
UniProt Protein Name
Calpain small subunit 2
UniProt Synonym Protein Names
Calcium-dependent protease small subunit 2
Protein Family
UniProt Gene Name
CAPNS2  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
CPNS2_HUMAN
UniProt Comments for CAPNS2
CAPNS2: Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. This small subunit may act as a tissue-specific chaperone of the large subunit, possibly by helping it fold into its correct conformation for activity.

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: cytoplasm; plasma membrane

Molecular Function: calcium-dependent cysteine-type endopeptidase activity; calcium ion binding

Biological Process: proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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