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CAS9 recombinant protein :: S. Pyogenes CRISPR-Associated Protein-9 Nuclease Recombinant Protein

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Catalog # MBS140642
Unit / Price
  0.05 mg  /  $790 +1 FREE 8GB USB
  0.1 mg  /  $1,320 +1 FREE 8GB USB
  0.5 mg  /  $3,900 +4 FREE 8GB USB
CAS9 recombinant protein
Product Name

S. Pyogenes CRISPR-Associated Protein-9 Nuclease (CAS9), Recombinant Protein

Also Known As

Recombinant S. Pyogenes CRISPR-Associated Protein-9 Nuclease

Product Synonym Names
CAS9 S. Pyogenes; CRISPR-Associated Protein-9 Nuclease S. Pyogenes Recombinant; CRISPR-associated endonuclease Cas9/Csn1; SpyCas9; cas9; csn1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
1368
3D Structure
ModBase 3D Structure for Q99ZW2
Host
E Coli
Purity/Purification
Greater than 97% as determined by SDS-PAGE.
Form/Format
The CAS9 solution (1mg/ml) contains 10mM Tris-Cl, 250mM NaCl, 0.1mM EDTA, 50% glycerol and 1mM DTT pH 7.5.
Sterile Filtered colorless solution.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Other Notes
Small volumes of CAS9 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CAS9 recombinant protein
Introduction: Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes, among other bacteria. S. Pyogenes utilizes Cas9 to remember and later probe and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. In case that the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In addition to its original role in bacterial immunity, the Cas9 protein has been heavily employed as a genome engineering tool to induce site-directed double strand breaks in DNA. These disruptions may lead to gene inactivation or the presentation of heterologous genes via non-homologous end joining and homologous recombination respectively in many laboratory model organisms.

Description: Recombinant Cas9-NLS comprises the entire Cas9 protein sequence (1368 amino acids) joined to a 6xHis tag at the N-terminus and a proprietary nuclear localization sequence (NLS) at the C-terminus. The protein has an apparent molecular weight of 160kDa on SDS-PAGE.
Product Categories/Family for CAS9 recombinant protein
NCBI/Uniprot data below describe general gene information for CAS9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
Molecular Weight
158,441 Da
NCBI Official Full Name
conserved hypothetical protein
NCBI Official Symbol
SPy_1046  [Similar Products]
NCBI Protein Information
hypothetical protein
UniProt Protein Name
CRISPR-associated endonuclease Cas9/Csn1
UniProt Synonym Protein Names
SpyCas9
UniProt Gene Name
UniProt Entry Name
CAS9_STRP1
UniProt Comments for CAS9
Cas9: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA probably requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA (By similarity). Cas9/crRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. Binding to dsDNA target requires a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif). When the CRISPR3/cas system consisting of cas9-cas1-cas2-csn2-CRISPR3 or just cas9-CRISPR3 is expressed in E.coli it prevents plasmids homologous to spacers 1 or 2 from transforming

Protein type: EC 3.1.-.-
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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