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Casp1 kit :: CASPASE-1 Kit

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Catalog # MBS239202
Unit / Price
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  100 Tests  /  $940 +1 FREE 8GB USB
Testing Data #1
Product Name

CASPASE-1 (Casp1), Kit

Full Product Name

FAM FLICA CASPASE-1 KIT

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Form/Format
Kit
Reagents In The Kits
4 vials of FAM-YVAD-FMK FLICA Reagent - lyophilized
10X Wash Buffer, 60 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst Stain, 1 mL
Preparation and Storage
Store the unopened kit (and each unopened component) at 4 degree C until the expiration date.
Protect the FLICA reagent from light at all times.
Once reconstituted, the 150X FLICA stock should be stored at -20 degree C protected from light.
Shelf Life: The 150X FLICA reconstituted stock is stable for up to 6 months and may be thawed twice during that time.
Product Note
Our Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Casp1 kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Casp1 kit
Description: The FAM FLICA Caspase 1 kit uses a quick and easy method to analyze active caspases in apoptotic cells.
Test Principle: Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.

The Caspase FLICA Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Applications Tested/Suitable for Casp1 kit
Flow cytometry (FC/FACS), Immunofluorescence (IF)

Testing Data #1 of Casp1 kit
Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases
Casp1 kit Testing Data #1 image
NCBI/Uniprot data below describe general gene information for Casp1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
45,576 Da[Similar Products]
NCBI Official Full Name
caspase-1
NCBI Official Synonym Full Names
caspase 1
NCBI Official Symbol
Casp1  [Similar Products]
NCBI Official Synonym Symbols
Ice; Il1bc
  [Similar Products]
NCBI Protein Information
caspase-1; CASP-1; IL-1 beta-converting enzyme; IL-1BC; Interleukin 1beta converting enzyme; interleukin 1, beta, convertase; interleukin-1 beta convertase; interleukin-1 beta-converting enzyme; p45
UniProt Protein Name
Caspase-1
UniProt Synonym Protein Names
Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45Cleaved into the following 2 chains:Caspase-1 subunit p20; Caspase-1 subunit p10
Protein Family
UniProt Gene Name
Casp1  [Similar Products]
UniProt Synonym Gene Names
Il1bc; Il1bce; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme  [Similar Products]
UniProt Entry Name
CASP1_RAT
NCBI Summary for Casp1
apoptotic protease that may play a role in inflammation and apoptosis in male sex organs [RGD, Feb 2006]
UniProt Comments for Casp1
CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis (By similarity)

Protein type: Apoptosis; Protease; EC 3.4.22.36
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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