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CASQ1 elisa kit :: Human calsequestrin 1 (fast-twitch, skeletal muscle) ELISA Kit

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Catalog # MBS925690
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Typical Testing Data/Standard Curve (for reference only)
Product Name

calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1), ELISA Kit

Also Known As

Human Calsequestrin-1, CASQ1 ELISA Kit

Product Synonym Names
Human Calsequestrin-1 (CASQ1) ELISA kit; RP11-536C5.6; CASQ; PDIB1; calmitine; calsequestrin 1; calsequestrin 1 (fast-twitch; skeletal muscle)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS925690 Testing Data
Sequence Length
396
OMIM
114250
3D Structure
ModBase 3D Structure for P31415
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human CASQ1. No significant cross-reactivity or interference between human CASQ1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
23.5 pg/ml -1500 pg/ml.
Sensitivity
The minimum detectable dose of human CASQ1 is typically less than 5.86 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CASQ1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCASQ1purchase
MBS925690 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CASQ1. The ELISA analytical biochemical technique of the MBS925690 kit is based on CASQ1 antibody-CASQ1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CASQ1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CASQ1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CASQ1 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CASQ1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CASQ1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CASQ1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CASQ1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CASQ1 elisa kit
CASQ1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS925690. Click to request current manual
NCBI/Uniprot data below describe general gene information for CASQ1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,160 Da
NCBI Official Full Name
calsequestrin-1
NCBI Official Synonym Full Names
calsequestrin 1 (fast-twitch, skeletal muscle)
NCBI Official Symbol
CASQ1  [Similar Products]
NCBI Official Synonym Symbols
CASQ; PDIB1
  [Similar Products]
NCBI Protein Information
calsequestrin-1; calmitin; calmitine; calsequestrin, skeletal muscle isoform
UniProt Protein Name
Calsequestrin-1
UniProt Synonym Protein Names
Calmitin; Calsequestrin, skeletal muscle isoform
Protein Family
UniProt Gene Name
CASQ1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
CASQ1_HUMAN
NCBI Summary for CASQ1
This gene encodes the skeletal muscle specific member of the calsequestrin protein family. Calsequestrin functions as a luminal sarcoplasmic reticulum calcium sensor in both cardiac and skeletal muscle cells. This protein, also known as calmitine, functions as a calcium regulator in the mitochondria of skeletal muscle. This protein is absent in patients with Duchenne and Becker types of muscular dystrophy. [provided by RefSeq, Jun 2013]
UniProt Comments for CASQ1
calsequestrin 1: Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. The skeletal muscle isoform (CASQ1) binds around 80 Ca(2+) ions, while the cardiac isoform (CASQ2) binds approximately 60 Ca(2+). Belongs to the calsequestrin family.

Protein type: Mitochondrial; Calcium-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: Golgi apparatus; I band; sarcoplasmic reticulum membrane; smooth endoplasmic reticulum; sarcoplasmic reticulum lumen; sarcoplasmic reticulum; mitochondrion; mitochondrial matrix; endoplasmic reticulum; T-tubule

Molecular Function: calcium ion binding

Biological Process: endoplasmic reticulum organization and biogenesis; protein polymerization; response to organic substance; regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; regulation of sequestering of calcium ion; skeletal muscle development; response to heat; sarcomere organization; response to denervation involved in regulation of muscle adaptation; transmembrane transport

Disease: Myopathy, Vacuolar, With Casq1 Aggregates
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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