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CASQ2 elisa kit :: Porcine Calsequestrin-2 (CASQ2) ELISA Kit

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Catalog # MBS7200165
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Calsequestrin-2 (CASQ2), ELISA Kit

Popular Item
Full Product Name

Porcine Calsequestrin-2 (CASQ2) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 611938
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of CASQ2. No significant cross-reactivity or interference between CASQ2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CASQ2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive (Quantitative)
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CASQ2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CASQ2purchase
MBS7200165 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calsequestrin-2 (CASQ2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CASQ2. The ELISA analytical biochemical technique of the MBS7200165 kit is based on CASQ2 antibody-CASQ2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CASQ2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CASQ2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CASQ2 elisa kit
Intended Uses: This CASQ2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CASQ2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CASQ2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CASQ2 antibody and an CASQ2-HRP conjugate. The assay sample and buffer are incubated together with CASQ2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CASQ2 concentration since CASQ2 from samples and CASQ2-HRP conjugate compete for the anti-CASQ2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CASQ2 from the sample, fewer sites are left to bind CASQ2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASQ2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CASQ2 elisa kit

Typical Testing Data/Standard Curve (for reference only) of CASQ2 elisa kit
CASQ2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7200165. Click to request current manual
NCBI/Uniprot data below describe general gene information for CASQ2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,436 Da
NCBI Official Full Name
calsequestrin-2
NCBI Official Synonym Full Names
calsequestrin 2 (cardiac muscle)
NCBI Official Symbol
CASQ2  [Similar Products]
NCBI Official Synonym Symbols
PDIB2
  [Similar Products]
NCBI Protein Information
calsequestrin-2; calsequestrin, cardiac muscle isoform; calsequestrin 2, fast-twitch, cardiac muscle
UniProt Protein Name
Calsequestrin-2
UniProt Synonym Protein Names
Calsequestrin, cardiac muscle isoform
Protein Family
UniProt Gene Name
CASQ2  [Similar Products]
UniProt Entry Name
CASQ2_HUMAN
NCBI Summary for CASQ2
The protein encoded by this gene specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. The protein is a calcium binding protein that stores calcium for muscle function. Mutations in this gene cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest. [provided by RefSeq, Jul 2008]
UniProt Comments for CASQ2
calsequestrin 2: Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. The skeletal muscle isoform (CASQ1) binds around 80 Ca(2+) ions, while the cardiac isoform (CASQ2) binds approximately 60 Ca(2+) ions. Defects in CASQ2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 2 (CPVT2); also known as stress-induced polymorphic ventricular tachycardia (VTSIP). CPVT2 is an autosomal recessive form of arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death. Belongs to the calsequestrin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: sarcoplasmic reticulum membrane; sarcoplasmic reticulum lumen; sarcoplasmic reticulum; junctional membrane complex; cytoplasm; voltage-gated calcium channel complex; intracellular; Z disc

Molecular Function: protein binding; protein homodimerization activity; calcium ion binding; calcium-dependent protein binding

Biological Process: protein polymerization; striated muscle contraction; regulation of heart rate; negative regulation of potassium ion transport; sequestering of calcium ion; sarcomere organization; detection of calcium ion; transmembrane transport; cardiac muscle contraction

Disease: Ventricular Tachycardia, Catecholaminergic Polymorphic, 1, With Or Without Atrial Dysfunction And/or Dilated Cardiomyopathy; Ventricular Tachycardia, Catecholaminergic Polymorphic, 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with CASQ2 elisa kit
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