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CaSR elisa kit :: Monkey Calcium Sensing Receptor ELISA Kit

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Catalog # MBS750510
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Calcium Sensing Receptor (CaSR), ELISA Kit

Full Product Name

Monkey Calcium Sensing Receptor ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene+612899
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Intended Uses
This CaSR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CaSR. This ELISA kit for research use only, not for therapeutic or diagnostic applications
!Intended Uses
This CaSR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CaSR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CaSR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CaSRpurchase
MBS750510 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calcium Sensing Receptor (CaSR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CaSR. The ELISA analytical biochemical technique of the MBS750510 kit is based on CaSR antibody-CaSR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CaSR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CaSR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CaSR elisa kit
Principle of the Assay: CaSR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CaSR antibody and an CaSR-HRP conjugate. The assay sample and buffer are incubated together with CaSR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CaSR concentration since CaSR from samples and CaSR-HRP conjugate compete for the anti-CaSR antibody binding site. Since the number of sites is limited, as more sites are occupied by CaSR from the sample, fewer sites are left to bind CaSR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CaSR concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CaSR elisa kit

Typical Testing Data/Standard Curve (for reference only) of CaSR elisa kit
CaSR elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS750510. Click to request current manual
NCBI/Uniprot data below describe general gene information for CaSR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
121,772 Da
NCBI Official Full Name
calcium sensing receptor, partial
NCBI Official Synonym Full Names
calcium-sensing receptor
NCBI Official Symbol
NCBI Official Synonym Symbols
CAR; FHH; FIH; HHC; EIG8; HHC1; NSHPT; PCAR1; GPRC2A; HYPOC1
  [Similar Products]
NCBI Protein Information
extracellular calcium-sensing receptor; parathyroid Ca(2+)-sensing receptor 1; parathyroid cell calcium-sensing receptor 1
UniProt Protein Name
Extracellular calcium-sensing receptor
UniProt Synonym Protein Names
Parathyroid cell calcium-sensing receptor 1; PCaR1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GPRC2A; PCAR1; CaSR; PCaR1  [Similar Products]
UniProt Entry Name
CASR_HUMAN
NCBI Summary for CaSR
The protein encoded by this gene is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism. [provided by RefSeq, Jul 2008]
UniProt Comments for CaSR
CaR: a G-protein coupled receptor that activates a phosphatidylinositol-calcium second messenger system. Functions as a calcium sensor for parathyroid and kidney to determine the extracellular calcium concentration and thus helps to maintain a stable calcium concentration. Two alternatively-spliced isoforms have been described.

Protein type: Membrane protein, integral; Receptor, GPCR; Motility/polarity/chemotaxis; Membrane protein, multi-pass; GPCR, family 3

Chromosomal Location of Human Ortholog: 3q13

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; phosphoinositide phospholipase C activity

Biological Process: cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; ossification; anatomical structure morphogenesis; metabolic process; chemosensory behavior; detection of calcium ion

Disease: Hypocalciuric Hypercalcemia, Familial, Type I; Epilepsy, Idiopathic Generalized, Susceptibility To, 8; Hyperparathyroidism, Neonatal Severe; Hypocalcemia, Autosomal Dominant 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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