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CBG elisa kit :: Human Corticosteroid Binding Globulin (CBG) ELISA Kit

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Catalog # MBS2703039 (SPECIAL PROMOTIONAL PRICING for a limited time)
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Corticosteroid Binding Globulin (CBG), ELISA Kit

Popular Item
Full Product Name

Human Corticosteroid Binding Globulin (CBG) ELISA Kit

Product Synonym Names
SERPINA6; Transcortin; Serpin A6; Serpin Peptidase Inhibitor,Clade A,Member 6(Alpha-1 Antiproteinase; Antitrypsin)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
J02943 mRNA
3D Structure
ModBase 3D Structure for P08185
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Corticosteroid Binding Globulin (CBG).
No significant cross-reactivity or interference between Corticosteroid Binding Globulin (CBG) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.073ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Corticosteroid Binding Globulin (CBG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Corticosteroid Binding Globulin (CBG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CBG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CBGpurchase
MBS2703039 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Corticosteroid Binding Globulin (CBG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CBG. The ELISA analytical biochemical technique of the MBS2703039 kit is based on CBG antibody-CBG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CBG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CBG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CBG elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corticosteroid Binding Globulin (CBG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Corticosteroid Binding Globulin (CBG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Corticosteroid Binding Globulin (CBG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corticosteroid Binding Globulin (CBG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CBG elisa kit
CBG elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2703039. Click to request current manual
NCBI/Uniprot data below describe general gene information for CBG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,141 Da
NCBI Official Full Name
corticosteroid-binding globulin
NCBI Official Synonym Full Names
serpin family A member 6
NCBI Official Symbol
SERPINA6  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
corticosteroid-binding globulin
UniProt Protein Name
Corticosteroid-binding globulin
UniProt Synonym Protein Names
Serpin A6; Transcortin
UniProt Gene Name
SERPINA6  [Similar Products]
UniProt Synonym Gene Names
CBG; CBG  [Similar Products]
NCBI Summary for CBG
This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events. [provided by RefSeq, Jul 2008]
UniProt Comments for CBG
Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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