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CBR1 elisa kit :: Human carbonyl reductase 1 ELISA Kit

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Catalog # MBS705832
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $550 +1 FREE 8GB USB
  96-Strip-Wells  /  $795 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

carbonyl reductase 1 (CBR1), ELISA Kit

Popular Item
Full Product Name

Human Carbonyl reductase [NADPH] 1, CBR1/CBR/CRN ELISA Kit

Product Synonym Names
Human Carbonyl reductase [NADPH] 1 (CBR1/CBR/CRN) ELISA kit; CBR; SDR21C1; hCBR1; carbonyl reductase (NADPH) 1; prostaglandin 9-ketoreductase; prostaglandin-E (2) 9-reductase; short chain dehydrogenase/reductase family 21C; member 1; carbonyl reductase 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS705832 Testing Data
OMIM
114830
3D Structure
ModBase 3D Structure for P16152
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human CBR1. No significant cross-reactivity or interference between human CBR1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Detection Range
31.25 pg/ml -2000 pg/mL
Sensitivity
<7.81 pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CBR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCBR1purchase
MBS705832 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the carbonyl reductase 1 (CBR1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CBR1. The ELISA analytical biochemical technique of the MBS705832 kit is based on CBR1 antibody-CBR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CBR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CBR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CBR1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CBR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CBR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CBR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CBR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CBR1 elisa kit
CBR1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS705832. Click to request current manual
NCBI/Uniprot data below describe general gene information for CBR1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30,375 Da
NCBI Official Full Name
carbonyl reductase
NCBI Official Synonym Full Names
carbonyl reductase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
CBR; hCBR1; SDR21C1
  [Similar Products]
NCBI Protein Information
carbonyl reductase [NADPH] 1; carbonyl reductase [NADPH] 1; carbonyl reductase (NADPH) 1; prostaglandin 9-ketoreductase; prostaglandin-E(2) 9-reductase; NADPH-dependent carbonyl reductase 1; 15-hydroxyprostaglandin dehydrogenase; short chain dehydrogenase/reductase family 21C, member 1
UniProt Protein Name
Carbonyl reductase [NADPH] 1
UniProt Synonym Protein Names
15-hydroxyprostaglandin dehydrogenase [NADP(+)] (EC:1.1.1.197); NADPH-dependent carbonyl reductase 1; Prostaglandin 9-ketoreductase; Prostaglandin-E(2) 9-reductase (EC:1.1.1.189)
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CBR; CRN  [Similar Products]
UniProt Entry Name
CBR1_HUMAN
NCBI Summary for CBR1
The protein encoded by this gene belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2013]
UniProt Comments for CBR1
Function: NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.4 PublicationsManual assertion based on experiment in:Ref.14

Catalytic activity: R-CHOH-R' + NADP+ = R-CO-R' + NADPH.

Enzyme regulation: Inhibited by quercetin, rutenin and its derivatives.2 PublicationsManual assertion based on experiment in:Ref.14
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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