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CCL17 elisa kit :: Human CCL17/TARC ELISA Kit

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Catalog # MBS175885
Unit / Price
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  96-Strip-Wells  /  $455 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CCL17/TARC, ELISA Kit

Full Product Name

Human CCL17/TARC PicoKine ELISA Kit

Product Synonym Names
C-C motif chemokine 17; chemokine (C-C motif) ligand 17; A 152E5.3; ABCD 2; C C motif chemokine 17; C-C motif chemokine 17; CC chemokine TARC; CCL 17; CCL17; CCL17_HUMAN; Chemokine (C C motif) ligand 17; Chemokine ligand 17; MGC138271; MGC138273; SCYA17; Small inducible cytokine A17; Small inducible cytokine subfamily A (Cys Cys) member 17; Small inducible cytokine subfamily A member 17; Small-inducible cytokine A17; T cell directed CC chemokine; Thymus and activation regulated chemokine; Thymus and activation-regulated chemokine
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
601520
3D Structure
ModBase 3D Structure for Q92583
Species Reactivity
Specificity
Natural and recombinant human CCL17
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 1pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CCL17 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CCL17purchase
MBS175885 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CCL17/TARC, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CCL17. The ELISA analytical biochemical technique of the MBS175885 kit is based on CCL17 antibody-CCL17 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCL17 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCL17. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CCL17 elisa kit
Principle of the assay: human CCL17 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL17 has been precoated onto 96-well plates. Standards (E.coli,A24-S94) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL17 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine (TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically bindsand induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2The genefor CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.3,4 Thestandard used in this kit is recombinant human CCL17, consisting of 71 amino acids with the molecular weight of 8Kda.

Typical Testing Data/Standard Curve (for reference only) of CCL17 elisa kit
Human CCL17/TARC PicoKine ELISA Kit standard curve
CCL17 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS175885. Click to request current manual
NCBI/Uniprot data below describe general gene information for CCL17. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
10,507 Da
NCBI Official Full Name
C-C motif chemokine 17
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 17
NCBI Official Symbol
CCL17  [Similar Products]
NCBI Official Synonym Symbols
TARC; ABCD-2; SCYA17; A-152E5.3
  [Similar Products]
NCBI Protein Information
C-C motif chemokine 17; CC chemokine TARC; T cell-directed CC chemokine; small-inducible cytokine A17; thymus and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 17
UniProt Protein Name
C-C motif chemokine 17
UniProt Synonym Protein Names
CC chemokine TARC; Small-inducible cytokine A17; Thymus and activation-regulated chemokine
Protein Family
UniProt Gene Name
CCL17  [Similar Products]
UniProt Synonym Gene Names
SCYA17; TARC  [Similar Products]
UniProt Entry Name
CCL17_HUMAN
NCBI Summary for CCL17
This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. The product of this gene binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells. [provided by RefSeq, Sep 2014]
UniProt Comments for CCL17
CCL17: Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; extracellular region

Molecular Function: CCR4 chemokine receptor binding; chemokine activity; receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; multicellular organismal development; immune response; chemotaxis; negative regulation of myoblast differentiation; inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with CCL17 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Mammary Gland Antibodies  >1 publications with CCL17 and Mammary Gland
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