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CCL28 elisa kit :: Mouse CCL28 ELISA Kit

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Catalog # MBS177311
Unit / Price
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  96-Strip-Wells  /  $425 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CCL28, ELISA Kit

Full Product Name

Mouse CCL28 PicoKine ELISA Kit

Product Synonym Names
C-C motif chemokine 28; chemokine (C-C motif) ligand 28; CCK 1; CCK1; CCL 28; Chemokine (C-C motif) ligand 28; MEC; MGC71902; Mucosae associated epithelial chemokine; OTTHUMP0000022162; SCYA28; Small inducible cytokine A28; Small inducible cytokine A28 [Precursor]; Small inducible cytokine subfamily A (Cys-Cys), member 28
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q9JIL2
Species Reactivity
Specificity
Natural and recombinant mouse CCL28
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CCL28 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CCL28purchase
MBS177311 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CCL28, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CCL28. The ELISA analytical biochemical technique of the MBS177311 kit is based on CCL28 antibody-CCL28 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCL28 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCL28. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CCL28 elisa kit
Principle of the assay: mouse CCL28 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL28 has been precoated onto 96-well plates. Standards (E.coli, I23-R130) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL28 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL28 amount of sample captured in plate.
Background: CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.

Typical Testing Data/Standard Curve (for reference only) of CCL28 elisa kit
Mouse CCL28 PicoKine ELISA Kit standard curve
CCL28 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS177311. Click to request current manual
NCBI/Uniprot data below describe general gene information for CCL28. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
14,570 Da
NCBI Official Full Name
C-C motif chemokine 28
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 28
NCBI Official Symbol
Ccl28  [Similar Products]
NCBI Official Synonym Symbols
MEC; CCK1; Scya28
  [Similar Products]
NCBI Protein Information
C-C motif chemokine 28; small inducible cytokine A28; small-inducible cytokine A28
UniProt Protein Name
C-C motif chemokine 28
UniProt Synonym Protein Names
Small-inducible cytokine A28
Protein Family
UniProt Gene Name
Ccl28  [Similar Products]
UniProt Synonym Gene Names
Scya28  [Similar Products]
UniProt Entry Name
CCL28_MOUSE
UniProt Comments for CCL28
CCL28: Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function: chemokine activity; cytokine activity

Biological Process: elevation of cytosolic calcium ion concentration; immune response; positive regulation of cell-matrix adhesion; chemotaxis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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