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CCL3 elisa kit :: Rat CCL3 ELISA Kit

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Catalog # MBS824889
Unit / Price
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  96 Tests  /  $470 +1 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

CCL3, ELISA Kit

Popular Item
Full Product Name

Rat CCL3 ELISA Kit

Product Synonym Names
G0S19-1; MIP1A; SCYA3; C-C motif chemokine 3; G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; PAT 464.1; SIS-beta; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
ModBase 3D Structure for P50229
Species Reactivity
Specificity
The Rat CCL3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat CCL3 proteins within the range of 7.8 pg/ml - 500 pg/ml.
Samples
Cell Culture Supernatants, Serum, Plasma
Assay Type
Quantitative Sandwich
Sensitivity
<1 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CCL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CCL3purchase
MBS824889 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CCL3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CCL3. The ELISA analytical biochemical technique of the MBS824889 kit is based on CCL3 antibody-CCL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CCL3 elisa kit
Principle of the Assay: MBS Rat CCL3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CCL3 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat CCL3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CCL3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.
Applications Tested/Suitable for CCL3 elisa kit
Sandwich ELISA (SE)

Typical Testing Data/Standard Curve (for reference only) of CCL3 elisa kit
CCL3 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS824889. Click to request current manual
NCBI/Uniprot data below describe general gene information for CCL3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
10,335 Da
NCBI Official Full Name
C-C motif chemokine 3
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 3
NCBI Official Symbol
NCBI Official Synonym Symbols
Scya3; MIP-1a
  [Similar Products]
NCBI Protein Information
C-C motif chemokine 3; MIP-1-alpha; small inducible cytokine A3; small-inducible cytokine A3; macrophage inflammatory protein 1-alpha; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3)
UniProt Protein Name
C-C motif chemokine 3
UniProt Synonym Protein Names
Macrophage inflammatory protein 1-alpha; MIP-1-alpha; Small-inducible cytokine A3
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Mip1a; Scya3; MIP-1-alpha  [Similar Products]
UniProt Entry Name
CCL3_RAT
NCBI Summary for CCL3
mediates monocyte and neutrophil chemotaxis; may play a role in the pathogenesis of acute lung injury [RGD, Feb 2006]
UniProt Comments for CCL3
Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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