• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CCR5 elisa kit :: Human Chemokine C-C-Motif Receptor 5 (CCR5) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2020083 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $255 +1 FREE 8GB USB
  48-Strip-Wells  /  $455 +1 FREE 8GB USB
  96-Strip-Wells  /  $610 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,470 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,445 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Chemokine C-C-Motif Receptor 5 (CCR5), ELISA Kit

Also Known As

Chemokine C-C-Motif Receptor 5 (CCR5) ELISA Kit

Product Synonym Names
CD195; CC-CKR5; CCCKR5; CMKBR5; CHEMR13; HIV-1 fusion coreceptor
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
352
OMIM
601373
3D Structure
ModBase 3D Structure for P51681
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Chemokine C-C-Motif Receptor 5 (CCR5).
No significant cross-reactivity or interference between Chemokine C-C-Motif Receptor 5 (CCR5) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
15.6-1000pg/mL
Sensitivity
< 5.9pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Chemokine C-C-Motif Receptor 5 (CCR5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Chemokine C-C-Motif Receptor 5 (CCR5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CCR5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCCR5purchase
MBS2020083 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Chemokine C-C-Motif Receptor 5 (CCR5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CCR5. The ELISA analytical biochemical technique of the MBS2020083 kit is based on CCR5 antibody-CCR5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCR5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCR5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CCR5 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Receptor 5 (CCR5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Receptor 5 (CCR5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Receptor 5 (CCR5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Receptor 5 (CCR5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CCR5 elisa kit
CCR5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2020083. Click to request current manual
NCBI/Uniprot data below describe general gene information for CCR5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,524 Da
NCBI Official Full Name
Chemokine (C-C motif) receptor 5
NCBI Official Synonym Full Names
chemokine (C-C motif) receptor 5 (gene/pseudogene)
NCBI Official Symbol
NCBI Official Synonym Symbols
CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
  [Similar Products]
NCBI Protein Information
C-C chemokine receptor type 5
UniProt Protein Name
C-C chemokine receptor type 5
UniProt Synonym Protein Names
CHEMR13; HIV-1 fusion coreceptor; CD_antigen: CD195
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CMKBR5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5  [Similar Products]
UniProt Entry Name
CCR5_HUMAN
NCBI Summary for CCR5
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2015]
UniProt Comments for CCR5
CCR5: a 7-transmembrane G-linked receptor for a number of inflammatory C-C type chemokines including MIP-1-alpha, MIP-1-beta and RANTES. Transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (along with CD4) for HIV-1 R5 isolates. Interacts with PRAF2. Interacts with HIV-1 surface protein gp120. Efficient ligand binding to CCL3/MIP-1alpha and CCR4/MIP-1beta requires sulfation, O-glycosylation and sialic acid modifications. Glycosylation on S6 is required for efficient binding of CCL4. Interacts with ADRBK1. Interacts with ARRB1 and ARRB2. Variations in CCR5 are associated with resistance or susceptibility to immunodeficiency virus type 1 (resistance or susceptibility to HIV-1). Variations in CCR5 gene also influence the rate of progression to AIDS after infection. R60S variant, a naturally occurring mutation in a conserved residue in the first intracellular domain of CCR5, results in reduced amounts of the protein in the membrane and consequently may be associated with reduced susceptibility to infection by microbes that depend on these molecules as their receptors. Variations in CCR5 are associated with susceptibility to West Nile virus (WNV) infection

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; GPCR, family 1; Receptor, cytokine

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cell surface; integral to plasma membrane; cytoplasm; plasma membrane; endosome; external side of plasma membrane

Molecular Function: protein binding; C-C chemokine receptor activity; chemokine receptor activity; C-C chemokine binding; coreceptor activity; actin binding; phosphoinositide phospholipase C activity

Biological Process: viral reproduction; calcium-mediated signaling; MAPKKK cascade; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; chemotaxis; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; cell-cell signaling; calcium ion transport; cellular defense response; dendritic cell chemotaxis; entry into host cell; immune response; inflammatory response

Disease: West Nile Virus, Susceptibility To; Diabetes Mellitus, Insulin-dependent, 22; Hepatitis C Virus, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000