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CD14 elisa kit :: Human CD14 molecule ELISA Kit

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Catalog # MBS704244 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $375 +1 FREE 8GB USB
  96-Strip-Wells  /  $535 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,295 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,330 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CD14 molecule, ELISA Kit

Popular Item
Full Product Name

Human soluble cluster of differentiation 14, sCD14 ELISA Kit

Product Synonym Names
Human soluble cluster of differentiation 14; sCD14 ELISA Kit; CD14 antigen; monocyte differentiation antigen CD14; Human soluble cluster of differentiation 14; sCD14
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS704244 Testing Data
OMIM
158120
3D Structure
ModBase 3D Structure for P08571
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human sCD14. No significant cross-reactivity or interference between human sCD14 and analogues was observed.
Samples
Serum, plasma, cell culture supernates, tissue homogenates, urine.
Assay Type
Quantitative Sandwich
Sensitivity
Typically less than 0.229 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CD14 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCD14purchase
MBS704244 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD14 molecule, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD14. The ELISA analytical biochemical technique of the MBS704244 kit is based on CD14 antibody-CD14 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD14 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD14. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CD14 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD14 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CD14 elisa kit
CD14 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS704244. Click to request current manual
NCBI/Uniprot data below describe general gene information for CD14. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,076 Da
NCBI Official Full Name
monocyte differentiation antigen CD14
NCBI Official Synonym Full Names
CD14 molecule
NCBI Official Symbol
NCBI Protein Information
monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
UniProt Protein Name
Monocyte differentiation antigen CD14
UniProt Synonym Protein Names
Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14Cleaved into the following 2 chains:Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form
UniProt Gene Name
UniProt Entry Name
CD14_HUMAN
NCBI Summary for CD14
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]
UniProt Comments for CD14
Function: In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.1 PublicationManual assertion based on experiment in:Ref.15
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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