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CD152 protein

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Catalog # MBS666790
Unit / Price
  50 Tests  /  $575 +1 FREE 8GB USB
Testing Data
Product Name

CD152, Protein

Also Known As

CD152(CTLA-4)-muIg-FITC

Product Synonym Names
Human CD152(CTLA-4) Ig/FITC Fusion Protein (also binds to mouse CD80/CD86)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Host
BHK cells
Species Reactivity
Human, Primate, Mouse, Pig, Cow, Cat
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
Volume: 200 ul (lot specific)
Molecular Structure
A soluble 110 kd dimeric fusion protein consisting of the extracellular (125aa) domain of human CD152 (CTLA-4) fused to murine IgG2a Fc
Transfectant Cell Line
BHK
Performance
Five x 105 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 mul of CD152 Ig/FITC at a 1:20 dilution factor. Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.78 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml CTLA-4 Ig Fusion Protein.
Conjugation
FITC
Production
Human CD152 Ig fusion protein from tissue culture supernatant of BHK transfectants was Protein A purified to >95% by SDS-PAGE (<1% bovine immunoglobulin), and reacted with FITC. Unconjugated FITC was removed from conjugate using a desalting column. The fusion protein/FITC conjugate is at 20 mug/ml with a Fluorescein/IgG molar ratio of 10.8.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Other Notes
Small volumes of CD152 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CD152 protein
Information: Immune response mediated by T cells can be characterized to functionally proceed as follows: antigen recognition by the T cell receptor, activation through costimulation, effector activities to eliminate antigen and finally down regulation. Human CD152 is a cell surface glycoprotein expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation. Therefore, CD152 may be an important player in down regulating T cell mediated immune responses. The CD152 Ig fusion protein has biological activity and binds with high affinity to human or mouse CD80 (B7-1) and CD86 (B7-2). CD152 Ig will block the binding of anti-CD80 (B7-1) and anti-CD86 (B7-2) monoclonal antibodies.
Applications Tested/Suitable for CD152 protein
Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for CD152 protein
1:20 (or use 4 mul of concentrated stock per 5 x 105-cell test)

Testing Data of CD152 protein
CD152 protein Testing Data image
Product References and Citations for CD152 protein
T. Lindsten, et al, (1993) J Immunol 151: 3489-3499. T.L. Walunas, et al, (1994) Immunity 1: 405-413. N.J. Karandikar, et al, (1996) J Exp Med 184: 783-788. A.H. Cross, et al, (1995) J Clin Invest 95: 2783-2789. P.A. Morton, et al, (1996) J Immunol 156: 1047-1054. Martin K. Oaks and Karen M. Hallett, (2000) J Immunol 164: 5015-5018. S.J. Fass, et al, (2000) J Immunol 164: 6340-6348.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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