• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD154 recombinant protein

Scan QR to view Datasheet
Catalog # MBS232306
Unit / Price
  0.01 mg  /  $505 +1 FREE 8GB USB
CD154 recombinant protein
Product Name

CD154, Recombinant Protein

Full Product Name

RECOMBINANT HUMAN CD154

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
300386
3D Structure
ModBase 3D Structure for P29965
Purity/Purification
>98% by SDS PAGE and HPLC sequence analysis
Form/Format
Purified recombinant protein - lyophilised
Concentration
Approximate Protein Concentration: 1.0 mg/ml (lot specific)
Reconstitution
Reconstitute with 10ul 5-10mM sodium phosphate pH7.5. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.
Other Notes
Small volumes of CD154 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CD154 recombinant protein
Recombinant Human CD154 is an E Coli derived protein corresponding to human CD154, also known as CD40 ligand, CD40L or TRAP. CD154 is a membrane protein expressed by activated T cells. Binding of CD154 to its ligand (CD40) on B cells induces B cell proliferation and immunoglobulin class switching.This preparation of Recombinant Human CD154 comprises the receptor binding TNF-like domain of human CD154.
Applications Tested/Suitable for CD154 recombinant protein
Functional Assays (FN), Western Blot (WB)
Application Notes for CD154 recombinant protein
Western Blotting: Minimum Dilution: 0.05 ug; Maximum Dilution: 0.5 ug
NCBI/Uniprot data below describe general gene information for CD154. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
Protein Molecular Weight: 16.3kDa (149 amino acid sequence)
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
CD40LG  [Similar Products]
NCBI Official Synonym Symbols
IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
  [Similar Products]
NCBI Protein Information
CD40 ligand; CD40 antigen ligand; CD40-L; T-B cell-activating molecule; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5
UniProt Protein Name
CD40 ligand
UniProt Synonym Protein Names
T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154Cleaved into the following 2 chains:CD40 ligand, membrane form; CD40 ligand, soluble form
UniProt Gene Name
CD40LG  [Similar Products]
UniProt Synonym Gene Names
CD40L; TNFSF5; TRAP; CD40-L; TRAP  [Similar Products]
UniProt Entry Name
CD40L_HUMAN
NCBI Summary for CD154
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
UniProt Comments for CD154
CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: Xq26

Cellular Component: extracellular space; integral to plasma membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: cytokine activity; CD40 receptor binding; tumor necrosis factor receptor binding

Biological Process: B cell proliferation; platelet activation; regulation of immune response; leukocyte adhesion; positive regulation of interleukin-12 production; B cell differentiation; immunoglobulin secretion; isotype switching; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis

Disease: Immunodeficiency With Hyper-igm, Type 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000