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CD16 recombinant protein :: CD16a (FcgR3a) Recombinant Protein

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Catalog # MBS553215
Unit / Price
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  0.02 mg  /  $220 +1 FREE 8GB USB
  0.1 mg  /  $540 +1 FREE 8GB USB
  0.5 mg  /  $1,915 +2 FREE 8GB USB
CD16 recombinant protein
Product Name

CD16a (FcgR3a), Recombinant Protein

Popular Item
Full Product Name

Human CD16a Recombinant (FcgR3a)

Product Synonym Names
Human CD16a Recombinant (FcgrR3a) ; cd16; cd-16; fcgr3a
Product Gene Name

CD16 recombinant protein

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
146740
3D Structure
ModBase 3D Structure for P08637
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant CD16a is supplied as a 0.2 um filtered PBS solution, pH7.2.
Domain
Ig-like C2
Source Note
Optimized DNA sequence encoding Human extracellular domain of human CD16a including a C-terminal His tag was expressed in HEK293 cells.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Biological Activity
Recombinant CD16a activity was measured by the ability to bind human IgG with an affinity constant of 180nM.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.
Molecular Weight Note
Recombinant CD16a is a monomer protein consisting of 203 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.
Preparation and Storage
Recombinant CD16a, as supplied, can be stored in working aliquots at 2-8°C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of CD16 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for
CD16 recombinant protein
Human CD16 is encoded by two genes, FcγRIIIA and FcγRIIIB. FcγRIIIa is a transmembranous isoform of the receptor expressed on the surface of natural killer (NK) cells, macrophages, a subset of monocytes and of T cells in association with dimers of the γ-chain of FcεRI and/or ζ-chain of T-cell receptors. FcγRIIIb is a glycosylphosphatidylinositol -anchored isoform restricted to neutrophils. FcγRIII plays a significant role in the clearance of immune complexes, antibody- dependent cellular cytotoxicity of K/NK cells,phagocytosis and antigen presentation.
Product Categories/Family for CD16 recombinant protein
Receptors CD
NCBI/Uniprot data below describe general gene information for CD16. It may not necessarily be applicable to this product.
NCBI GI #
50726979
NCBI GeneID
2214
NCBI Accession #
NP_000560.5 [Other Products]
NCBI GenBank Nucleotide #
NM_000569.6 [Other Products]
UniProt Primary Accession #
P08637 [Other Products]
UniProt Secondary Accession #
Q53FJ0; Q53FL6; Q5EBR4; Q65ZM6; Q6PIJ0; A2N6W9[Other Products]
UniProt Related Accession #
P08637; Q9ULV2; Q9UN20[Other Products]
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NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor III-A isoform a
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-A
UniProt Synonym Protein Names
CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2
UniProt Gene Name
FCGR3A  [Similar Products]
UniProt Synonym Gene Names
CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa  [Similar Products]
UniProt Entry Name
FCG3A_HUMAN
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NCBI Summary for CD16
This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD16
FCGR3A: Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: IgG binding

Biological Process: regulation of immune response; innate immune response; immune response

Disease: Immunodeficiency 20
Product References and Citations for CD16 recombinant protein
• affinity and kinetic analysis of fc receptor iiia (CD16a) binding to igg ligands .J. Biol. Chem., Mar 2007; 282: 6210 - 6221.
• phagocytosis stimulates mobilization and shedding of intracellular CD16a in human monocytes and macrophages: inhibition by hiv-1 infection .J. Leukoc. Biol., Feb 2006; 79: 294 - 302.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CD16 recombinant protein
 Reference Product  PubMed Publications
 FCGR1A recombinant protein  >6 publications with CD16 and FCGR1A
 CD4 recombinant protein  >4 publications with CD16 and CD4
 PTPRC recombinant protein  >1 publications with CD16 and PTPRC
Pathways associated with CD16 recombinant protein
 Products by Pathway  Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies  Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Natural Killer Cell Mediated Cytotoxicity Pathway antibodies  Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
 Natural Killer Cell Mediated Cytotoxicity Pathway antibodies  Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
Diseases associated with CD16 recombinant protein
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >57 publications with CD16 and Neoplasms
 Autoimmune Diseases Antibodies  >52 publications with CD16 and Autoimmune Diseases
 Lymphoma Antibodies  >32 publications with CD16 and Lymphoma
 Arthritis, Rheumatoid Antibodies  >26 publications with CD16 and Arthritis, Rheumatoid
 Lymphoma, Non-Hodgkin Antibodies  >24 publications with CD16 and Lymphoma, Non-Hodgkin
 Lupus Erythematosus, Systemic Antibodies  >18 publications with CD16 and Lupus Erythematosus, Systemic
 Intestinal Diseases Antibodies  >16 publications with CD16 and Intestinal Diseases
 Necrosis Antibodies  >16 publications with CD16 and Necrosis
 Inflammation Antibodies  >9 publications with CD16 and Inflammation
 Leukemia Antibodies  >8 publications with CD16 and Leukemia
Organs/Tissues associated with CD16 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >89 publications with CD16 and Blood
 Ovary Antibodies  >5 publications with CD16 and Ovary
 Kidney Antibodies  >4 publications with CD16 and Kidney
 Lung Antibodies  >3 publications with CD16 and Lung
 Liver Antibodies  >2 publications with CD16 and Liver
 Brain Antibodies  >1 publications with CD16 and Brain
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