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CD209 elisa kit :: Human CD209 antigen (CD209) ELISA Kit

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Catalog # MBS7212702
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CD209 antigen (CD209), ELISA Kit

Popular Item
Also Known As

Human CD209 antigen (CD209) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
614371
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of CD209. No significant cross-reactivity or interference between CD209 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD209 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CD209 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCD209purchase
MBS7212702 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD209 antigen (CD209) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD209. The ELISA analytical biochemical technique of the MBS7212702 kit is based on CD209 antibody-CD209 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD209 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD209. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CD209 elisa kit
Intended Uses: This CD209 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CD209. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CD209 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD209 antibody and an CD209-HRP conjugate. The assay sample and buffer are incubated together with CD209-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD209 concentration since CD209 from samples and CD209-HRP conjugate compete for the anti-CD209 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD209 from the sample, fewer sites are left to bind CD209-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD209 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CD209 elisa kit

Typical Testing Data/Standard Curve (for reference only) of CD209 elisa kit
CD209 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7212702. Click to request current manual
NCBI/Uniprot data below describe general gene information for CD209. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
33,874 Da
NCBI Official Full Name
CD209 antigen isoform 8
NCBI Official Synonym Full Names
CD209 molecule
NCBI Official Symbol
CD209  [Similar Products]
NCBI Official Synonym Symbols
CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
  [Similar Products]
NCBI Protein Information
CD209 antigen; HIV gpl20-binding protein; C-type lectin domain family 4 member L; C-type lectin domain family 4, member L; dendritic cell-specific ICAM-3-grabbing non-integrin 1; dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin
UniProt Protein Name
CD209 antigen
UniProt Synonym Protein Names
C-type lectin domain family 4 member L; Dendritic cell-specific ICAM-3-grabbing non-integrin 1; DC-SIGN; DC-SIGN1; CD_antigen: CD209
Protein Family
UniProt Gene Name
CD209  [Similar Products]
UniProt Synonym Gene Names
CLEC4L; DC-SIGN; DC-SIGN1  [Similar Products]
UniProt Entry Name
CD209_HUMAN
NCBI Summary for CD209
This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.[provided by RefSeq, Feb 2009]
UniProt Comments for CD209
CD209: Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri- mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA. Homotetramer. Binds to many viral surface glycoproteins such as HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus envelope glycoproteins, cytomegalovirus gB, HCV E2 and dengue virus major envelope protein E. Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes. 13 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: cell surface; membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: mannose binding; protein binding; peptide antigen binding; metal ion binding; virion binding; carbohydrate binding

Biological Process: cell-cell recognition; heterophilic cell adhesion; antigen processing and presentation; leukocyte adhesion; virus-host interaction; regulation of T cell proliferation; innate immune response; peptide antigen transport; endocytosis; viral genome replication; virion attachment to host cell surface receptor

Disease: Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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