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CD252 protein

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Catalog # MBS666846
Unit / Price
  0.025 mg  /  $575 +1 FREE 8GB USB
Testing Data
Product Name

CD252, Protein

Popular Item
Also Known As

CD252(OX40L)-muCD8-Biotin

Product Synonym Names
Human CD252(CD134L)-muCD8/Biotin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Host
CHO cells
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
0.5 mg/ml (lot specific)
Molecular Structure
A soluble molecule consisting of the extracellular domain (132 aa) of human CD252 fused to the extracellular domain (167aa) of murine CD8 alpha.
Transfectant Cell Line
CHO
Performance
Five x 105 cultured human HPB-MLT cells per tube were washed and incubated 45 minutes on ice with 80 ul of CD252-muCD8/Biotin at a concentration of 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.9 log10 fluorescent units when compared to a buffer control. Binding was blocked when reagent was pre incubated with anti-CD252 antibody at a concentration of 100 ug/ml.
Conjugation
Biotin
Production
Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using affinity and size exclusion chromatography), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Other Notes
Small volumes of CD252 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CD252 protein
Information: Human CD252 (CD134L, OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD252 is involved with costimulatory activity between B cells and T cells (1). CD252-muCD8 fusion protein binds to CD134 positive cells in Flow cytometry.
Applications Tested/Suitable for CD252 protein
Flow Cytometry (FC/FACS), ELISA (EIA)

Testing Data of CD252 protein
CD252 protein Testing Data image
Product References and Citations for CD252 protein
1. W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763. 2. L.M. Higgins, et al, (1999) J Immunol 162: 486-493. 3. A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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