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CD273 protein

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Catalog # MBS666852
Unit / Price
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  0.025 mg  /  $575 +1 FREE 8GB USB
Testing Data
Product Name

CD273, Protein

Full Product Name

CD273-muIg-Biotin

Product Synonym Names
Human CD273(B7-DC,)-muIg/Biotin Fusion Protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
(221)gtepr gptikpcppc kcpapnllgg psvfifppki kdvlmislsp ivtcvvvdvs eddpdvqisw fvnnvevhta qtqthredyn stlrvvsalp iqhqdwmsgk efkckvnnkd lpapiertis kpgsvrapqv yvlpppeeem tkkqvtvtcm vtdfmpediy vewtnngkte lnykntepvl dsdgsyfmys klgvekknwv ernsyscsvv heglhnhhtt ksfsrtpg(4 55) Predic ted monomeric (non glycosylat ed) molecular weight: 49 kd. The molecule is dimeric and runs at ~135 kd in SDS-PAGE under native conditions , and ~74 kd reduced
(20)lftvt vpkelyiieh gsnvtlecnf dtgshvnlga itaslqkven dtsphrerat lleeqlplgk asfhipqvqv rdegqyqcii iygvawdyky ltlkvkasyr kinthilkvp etdeveltcq atyplaevsw pnvsvpants hsrtpeglyq vtsvlrlkpp pgrnfscvfw nthvreltla sidlqsqmep rthpt (220 )
Host
CHO cells
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
0.5 mg/ml (lot specific)
Transfectant Cell Line
CHO
Performance
CD273-muIg/Biotin is functional in EIA as a detection reagent binding to plate-coated recombinant CD279(PD-1). Streptavidin/HRP was used as a secondary detector and TMB/H2 O2 as a chromagen/substrate.
Conjugation
Biotin
Production
Human CD274-muIg fusion protein was Protein A purified from (low FBS containing) tissue culture supernatant of CHO transfectants, and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate by desalting column.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Product should retain activity for at least 6 months after shipping date when stored as recommended.
Other Notes
Small volumes of CD273 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CD273 protein
Information: CD273 (B7-DC, PCDL-2, Programmed cell death ligand 2, Butyrophilin-like protein) is a type I surface molecule with homology to CD80, CD86, CD274. It is expressed primarily by Dendritic cells.and provides a stimulatory signal to CD279 (PD-1, Programmed Death molecule) which serves an important immunoregulatory role by down regulating T cell response. CD273 binds to CD279(PD-1) with a 2- 6 fold higher affinity than CD274(2). Recombinant CD273-muIg binds to recombinant CD279 in EIA.
Applications Tested/Suitable for CD273 protein
ELISA (EIA)

Testing Data of CD273 protein
CD273 protein Testing Data image
Product References and Citations for CD273 protein
1) E N Rozali, W J Lesterhuis, et al. (2012) Clin Develop Immunol 2012: 656340. 2) P Youngnak, H Konozo, et al. (2003) Biochemical and Biophysical Research Communications 307(3): 672-677.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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