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CD274 protein

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Catalog # MBS666818
Unit / Price
  0.025 mg  /  $575 +1 FREE 8GB USB
CD274 protein
Product Name

CD274, Protein

Popular Item
Full Product Name

CD274 (B7-H1)-muIg-Biotin

Product Synonym Names
Human CD274(B7-H1)-muIg/Biotin Fusion Protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MuCD8 alpha signal peptide residual amino acids + linker:(1) kpqapelrgs as

CD274 mature EC: (224aa): (13)ftvtvp kdlyvveygs nmtieckfpv ekqldlaali vywemedkni iqfvhgeedl kvqhssyrqr arllkdqlsl gnaalqitdv klqdagvyrc misyggadyk ritvkvnapy nkinqrilvv dvtseheltc qaegypkaev iwtssdhqvl sgkttttnsk reeklfnvts tlrintttne ifyctfrrld peenhtaelv ipelplahpp nerthtr

Linke r + Murine IgG2a Hinge + Fc (235aa):(237)gte prgptikpcp pckcpapnll ggpsvfifpp kikdvlmisl spivtcvvvd vseddpdvqi swfvnnvevh taqtqthred ynstlrvvsa lpiqhqdwms gkefkckvnn kdlpapiert iskpgsvrap qvyvlpppee emtkkqvtlt cmvtdfmped iyvewtnngk telnykntep vldsdgsyfm ysklrvekkn wvernsyscs vvheglhnhh ttksfsrtpg (471)
OMIM
605402
Host
CHO cells
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
0.5 mg/ml (lot specific)
Molecular Structure
A soluble fusion protein consisting of the murine CD8 alpha leader sequence, the mature extracellular (224aa) domain of human CD274 fused to murine IgG2a Fc + hinge (233aa).
Transfectant Cell Line
CHO
Performance
Purity of recombinant protein was >90% by SDS-PAGE. N-terminal sequence was as predicted: KPQAP. Biotinylated recombinant protein was active in EIA, binding to anti-CD274 mAbs ANC6H1, clone M1H1, and recombinant CD279(PD1).

Predicted monomeric (non glycosylated) molecular weight: 54.4kd. The molecule is dimeric and runs at about 120 kd in SDS-PAGE under native conditions.
Conjugation
Biotin
Production
Human CD27-muIg fusion protein was Protein A purified from (low FBS containing) tissue culture supernatant of CHO transfectants, and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate by desalting column.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Molecular Weight
Predicted monomeric (non glycosylated) molecular weight: 54.4kd.
The molecule is dimeric and runs at about 120 kd in SDS-PAGE under native conditions.
Buffer
50mM Sodium Phosphate pH7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended
Product should retain activity for at least 6 months after shipping date when stored as recommended.
Other Notes
Small volumes of CD274 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CD274 protein
Information: CD274 (B7-H1, PD-L1, Programmed Death Ligand) is a member of the B7 family and is expressed on a variety of tissues including lymphoid cells. It plays an important role in regulation of T cell activation, and is involved in progression of cancer, arthritis and HIV infection (3). CD274 binding to its receptor CD279 (PD-1) on activated T cells can decrease proliferation. Conversely, ligation of CD279 on primed T cells can stimulate IL-10 production. High levels of CD274 present in Renal cell carcinoma are associated with poor prognosis (1). Tumor expressed CD274 can increase apoptosis of tumor specific T cells resulting in better tumor cell survival (2). Gamma Interferon and PHA can up regulate CD274 expression on T cells.
Applications Tested/Suitable for CD274 protein
ELISA (EIA)
NCBI/Uniprot data below describe general gene information for CD274. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
CD274, partial
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274  [Similar Products]
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
  [Similar Products]
NCBI Protein Information
programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Synonym Protein Names
B7 homolog 1; B7-H1; CD_antigen: CD274
Protein Family
UniProt Gene Name
CD274  [Similar Products]
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1  [Similar Products]
UniProt Entry Name
PD1L1_HUMAN
UniProt Comments for CD274
CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: integral to membrane; plasma membrane; endomembrane system; external side of plasma membrane

Molecular Function: protein binding

Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; T cell costimulation; positive regulation of T cell proliferation; immune response; signal transduction
Product References and Citations for CD274 protein
1)Cancer Research (April 2006) 66:3381. 2)J Molecular Medicine (Feb 2004) 81(5):281. 3)Int J Hematol (Nov 2003) 78(4):321.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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