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CD278 protein

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Catalog # MBS666816
Unit / Price
  0.025 mg  /  $470 +1 FREE 8GB USB
CD278 protein
Product Name

CD278, Protein

Also Known As

CD278(ICOS)-muIg-Purified Preservative Free

Product Synonym Names
Human ICOS-muIg/R-PE Fusion Protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Host
CHO cells
Species Reactivity
Human
Purity/Purification
Purified Preservative Free
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Concentration
0.5 mg/ml (lot specific)
Molecular Structure
A soluble molecule consisting of the extracellular (121aa) domain of mature human ICOS fused to the murine IgG2a Fc (232 aa). Predicted monomeric weight is 42.8 kd (amino acid composition only).
Transfectant Cell Line
CHO
Performance
ICOS-muIg fusion protein binds to Raji cells in FACS. Five x 105 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 ul of ICOS-muIg at 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.42 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Binding was blocked when reagent was pre incubated with 100 ug/ml of recombinant GL50(ICOSL)-muIg. ICOS-muIg fusion protein was reactive in EIA using a Goat-anti-mouse-Ig antibody capture, followed by detection using GL50-muIg/Biotin and Streptavidin/HRP. Amino acid sequence of fusion protein was confirmed by n-terminal analysis (EINGS).
Production
Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using size exclusion chromatography. Product was 0.2u sterile-filtered and vialed under aseptic conditions.
Buffer
50 mM Sodium Phosphate pH 7.6, 100mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
Preparation and Storage
Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Other Notes
Small volumes of CD278 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CD278 protein
Information: The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Each secondary signal from CD28 or ICOS results in a discrete cytokine secretion profile displayed by the activated T cell.1 Both activation processes are effectively down regulated by CD152 (CTLA-4) engagement.2 Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be a ligand for ICOS.3 ICOS-muIg recombinant protein binds to recombinant GL50-muIg in EIA.
Applications Tested/Suitable for CD278 protein
Flow Cytometry (FC/FACS), ELISA (EIA)
Product References and Citations for CD278 protein
Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717. 2 Riley, J.L., C.H. June, et al. 2001, J. Immunol. 166: 4943-4948. 3 Ling, V., M. Collins, et al. 2000, J. Immunol. 164: 1653-1657. 4 Ling, V., M. Collins, et al. 2001, J. Immunol. 166: 7300-7308. 5 A.J. McAdam, et al, (2000) J Immunol 165: 5035-5040.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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