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CD28 elisa kit :: Human CD28 molecule ELISA Kit

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Catalog # MBS701080
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CD28 molecule, ELISA Kit

Popular Item
Full Product Name

human soluble cluster of differentiation 28, sCD28 ELISA Kit

Product Synonym Names
human soluble cluster of differentiation 28; sCD28 ELISA Kit; MGC138290; Tp44; CD28 antigen; CD28 antigen (Tp44) ; T-cell-specific surface glycoprotein; CD28 molecule
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS701080 Testing Data
OMIM
186760
3D Structure
ModBase 3D Structure for P10747
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human sCD28. No significant cross-reactivity or interference between human sCD28 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.625 ng/ml -40 ng/ml.
Sensitivity
The minimum detectable dose of human sCD28 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CD28 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCD28purchase
MBS701080 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD28 molecule, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD28. The ELISA analytical biochemical technique of the MBS701080 kit is based on CD28 antibody-CD28 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD28 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD28. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CD28 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD28 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD28 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD28 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD28 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CD28 elisa kit
CD28 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS701080. Click to request current manual
NCBI/Uniprot data below describe general gene information for CD28. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,066 Da
NCBI Official Full Name
T-cell-specific surface glycoprotein CD28 isoform 2
NCBI Official Synonym Full Names
CD28 molecule
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
T-cell-specific surface glycoprotein CD28; CD28 antigen
UniProt Protein Name
T-cell-specific surface glycoprotein CD28
UniProt Synonym Protein Names
TP44; CD_antigen: CD28
UniProt Gene Name
UniProt Entry Name
CD28_HUMAN
NCBI Summary for CD28
The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
UniProt Comments for CD28
Function: Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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