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CD3 dual color reagent :: CD3/CD19 Dual Color Reagent

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Catalog # MBS238059
Unit / Price
  50 Tests  /  $465 +1 FREE 8GB USB
Testing Data #1
Product Name

CD3/CD19, Dual Color Reagent

Full Product Name

MOUSE ANTI HUMAN CD3:FITC/CD19:RPE

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
186740
Isotype
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)
Clone Number
UCHT1/LT19
Host
Mouse
Specificity
UCHT1 recognises the human CD3 antigen, a complex of 5 polypeptide chains with molecular weights of 16-28kD, expressed by mature T cells.LT19 recognises the human CD19 antigen, a pan B cell marker.

This item may be used to simultaneously enumerate CD3 and CD19 positive cells.
Form/Format
FITC/RPE
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised
Reconstitution
Reconstitute with 0.5 ml distilled water
Perservative Stabilisers
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Other Notes
Small volumes of CD3 dual color reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CD3 dual color reagent
UCHT1 recognises the human CD3 antigen, a complex of 5 polypeptide chains with molecular weights of 16-28kD, expressed by mature T cells. LT19 recognises the human CD19 antigen, a pan B cell marker. MBS238059 may be used to simultaneously enumerate CD3 and CD19 positive cells.
Applications Tested/Suitable for CD3 dual color reagent
Flow cytometry (FC/FACS)
Application Notes for CD3 dual color reagent
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry: Maximum Dilution: Neat

Testing Data #1 of CD3 dual color reagent
MBS238059 staining of human lymphocytes
CD3 dual color reagent Testing Data #1 image
NCBI/Uniprot data below describe general gene information for CD3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,469 Da
NCBI Official Full Name
T-cell surface glycoprotein CD3 gamma chain
NCBI Official Synonym Full Names
CD3g molecule, gamma (CD3-TCR complex)
NCBI Official Symbol
NCBI Official Synonym Symbols
T3G; IMD17; CD3-GAMMA
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NCBI Protein Information
T-cell surface glycoprotein CD3 gamma chain; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 gamma chain
UniProt Protein Name
T-cell surface glycoprotein CD3 gamma chain
UniProt Synonym Protein Names
T-cell receptor T3 gamma chain; CD_antigen: CD3g
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
CD3G_HUMAN
NCBI Summary for CD3
The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008]
UniProt Comments for CD3
CD3G: The CD3 complex mediates signal transduction.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; alpha-beta T cell receptor complex

Molecular Function: transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; T cell receptor binding

Biological Process: protein transport; regulation of immune response; cell surface receptor linked signal transduction; T cell activation; T cell costimulation; establishment and/or maintenance of cell polarity; innate immune response; protein complex assembly; T cell receptor signaling pathway

Disease: Immunodeficiency 17
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with CD3 dual color reagent
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