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CD3 dual color reagent :: CD3/CD45RA Dual Color Reagent

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Catalog # MBS238087
Unit / Price
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  100 Tests  /  $485 +1 FREE 8GB USB
Testing Data #1
Product Name

CD3/CD45RA, Dual Color Reagent

Full Product Name

MOUSE ANTI RAT CD3:FITC/CD45RA:RPE

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Isotype
FITC reagent: IgM (MOUSE)
RPE reagent: IgG1 (MOUSE)
Clone Number
IF4/OX-33
Host
Mouse
Specificity
This item is a dual colour reagent recognising rat CD3 and CD45RA cell surface antigens.

Clone IF4 recognises rat CD3, a cell surface antigen expressed by mature lymphocytes.

Clone OX-33 recognises rat CD45RA, a cell surface antigen expressed by B-lymphocytes.
Form/Format
FITC/RPE
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
Reconstitution
Reconstitute with 1 ml distilled water
Perservative Stabilisers
Buffer Solution
Phosphate buffered saline
Target Species
Rat
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Other Notes
Small volumes of CD3 dual color reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CD3 dual color reagent
MBS238087 is a dual colour reagent recognising rat CD3 and CD45RA cell surface antigens. Clone IF4 recognises rat CD3, a cell surface antigen expressed by mature lymphocytes. Clone OX-33 recognises rat CD45RA, a cell surface antigen expressed by B-lymphocytes.
Applications Tested/Suitable for CD3 dual color reagent
Flow cytometry (FC/FACS)
Application Notes for CD3 dual color reagent
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cell in 100ul.
Flow Cytometry: Maximum Dilution: Neat

Testing Data #1 of CD3 dual color reagent
Staining of rat spleen cells with Mouse anti Rat CD3:FITC/CD45RA:RPE (MBS238087)
CD3 dual color reagent Testing Data #1 image
NCBI/Uniprot data below describe general gene information for CD3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
134,182 Da
NCBI Official Full Name
receptor-type tyrosine-protein phosphatase C isoform 3
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol
Ptprc  [Similar Products]
NCBI Official Synonym Symbols
Lca; RT7; CD45; L-CA; T200
  [Similar Products]
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C; Protein tyrosine phosphatase, receptor-type, c polypeptide; T200 glycoprotein; leucocyte common antigen; leukocyte common antigen A; leukocyte common antigen B
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Synonym Protein Names
Leukocyte common antigen; L-CA; T200; CD_antigen: CD45
UniProt Gene Name
Ptprc  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
PTPRC_RAT
NCBI Summary for CD3
member of a family of heavily glycosylated leukocyte cell surface glycoproteins; displays extensive O-glycosylation [RGD, Feb 2006]
UniProt Comments for CD3
CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.

Protein type: EC 3.1.3.48; Membrane protein, integral; Receptor protein phosphatase, tyrosine; Motility/polarity/chemotaxis

Cellular Component: internal side of plasma membrane; cell surface; focal adhesion; membrane; integral to plasma membrane; integral to membrane; plasma membrane; intracellular; cytosol; external side of plasma membrane; lipid raft

Molecular Function: heparin binding; heparan sulfate proteoglycan binding; protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase regulator activity; protein kinase binding

Biological Process: B cell proliferation; positive regulation of isotype switching to IgG isotypes; activation of MAPK activity; regulation of humoral immune response mediated by circulating immunoglobulin; regulation of cell cycle; positive regulation of T cell mediated cytotoxicity; protein amino acid dephosphorylation; T cell receptor signaling pathway; T cell proliferation; leukocyte adhesion; B cell receptor signaling pathway; positive regulation of MAPKKK cascade; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; positive regulation of T cell mediated immunity; response to gamma radiation; positive regulation of T cell proliferation; negative regulation of protein amino acid autophosphorylation; negative regulation of cytokine and chemokine mediated signaling pathway; positive regulation of gamma-delta T cell differentiation; defense response to virus; T cell differentiation; positive thymic T cell selection; regulation of B cell receptor signaling pathway; negative regulation of peptidyl-tyrosine phosphorylation; positive regulation of humoral immune response mediated by circulating immunoglobulin; substrate-bound cell migration, cell release from substrate; negative thymic T cell selection; positive regulation of antigen receptor-mediated signaling pathway; bone marrow development; heterotypic cell-cell adhesion; stem cell development; positive regulation of T cell differentiation; dephosphorylation; B cell differentiation; release of sequestered calcium ion into cytosol; regulation of B cell differentiation; negative regulation of protein kinase activity; positive regulation of alpha-beta T cell proliferation; hemopoietic progenitor cell differentiation; immunoglobulin biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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