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CD30L elisa kit :: Human CD30L ELISA Kit

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Catalog # MBS2508844
Unit / Price
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  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CD30L, ELISA Kit

Popular Item
Full Product Name

Human CD30L (Cluster of Differentiation 30 Lligand) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
603875
3D Structure
ModBase 3D Structure for P32971
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human CD30L. No significant cross-reactivity or interference between Human CD30L and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CD30L and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
7.813-500pg/mL
Sensitivity
Min: 4.688pg/mL; Max: 500pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CD30L elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CD30Lpurchase
MBS2508844 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD30L, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD30L. The ELISA analytical biochemical technique of the MBS2508844 kit is based on CD30L antibody-CD30L antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD30L antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD30L. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CD30L elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CD30L in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD30L. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CD30L and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD30L, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CD30L. You can calculate the concentration of Human CD30L in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CD30L elisa kit
CD30L elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2508844. Click to request current manual
NCBI/Uniprot data below describe general gene information for CD30L. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
26,017 Da
NCBI Official Full Name
CD30L protein
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 8
NCBI Official Symbol
TNFSF8  [Similar Products]
NCBI Official Synonym Symbols
CD153; CD30L; CD30LG
  [Similar Products]
NCBI Protein Information
tumor necrosis factor ligand superfamily member 8; CD153 antigen; CD30 antigen ligand; CD30 ligand; CD30-L
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 8
UniProt Synonym Protein Names
CD30 ligand; CD30-L; CD_antigen: CD153
UniProt Gene Name
TNFSF8  [Similar Products]
UniProt Synonym Gene Names
CD30L; CD30LG; CD30-L  [Similar Products]
UniProt Entry Name
TNFL8_HUMAN
NCBI Summary for CD30L
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
UniProt Comments for CD30L
TNFSF8: Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. Belongs to the tumor necrosis factor family.

Protein type: Membrane protein, integral; Cytokine; Apoptosis

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: extracellular space; integral to plasma membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; defense response to Gram-positive bacterium; CD8-positive, alpha-beta T cell differentiation; cell-cell signaling; apoptosis; immune response; positive regulation of transcription from RNA polymerase II promoter; signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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