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anti-CD32 antibody :: anti-Human CD32 Antibody

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Catalog # MBS855849
Unit / Price
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  0.1 mg  /  $215 +1 FREE 8GB USB
Western Blot (WB)
Product Name

CD32, Antibody

Full Product Name

CD32 (Phospho-Tyr292) Antibody

Product Synonym Names
CD32; CDW32; FC gamma receptor IIB; FC-gamma RII; FC-gamma RII-B; FC-gamma-RIIB; FCG2; FCG2B; FCGR2; FCGR2B; FCRII; FCgammaRIIB; IGFR2; IGG FC receptor II beta; IGG FC receptor II-B; LY-17; Low affinity immunoglobulin gamma FC region receptor II precursor; Low affinity immunoglobulin gamma FC region receptor II-B precursor
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
152700
3D Structure
ModBase 3D Structure for P31994
Species Reactivity
Human
Specificity
CD32 (Phospho-Tyr292) antibody detects endogenous levels of CD32 only when phosphorylated at tyrosine 292.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human CD32around the phosphorylation site of tyrosine 292 (I-T-YP-S-L).
All Sites
Human: Tyr292
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-CD32 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD32 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-CD32 antibody
WB: 1:500-1:1000
ELISA: 1:1000

Western Blot (WB) of anti-CD32 antibody
Western blot analysis of extracts from K562 cells, treated with PMA (125ng/ml, 30mins), using CD32 (Phospho-Tyr292) antibody.
anti-CD32 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CD32. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
31,873 Da
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor II-b
UniProt Synonym Protein Names
CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; CD_antigen: CD32
Protein Family
UniProt Gene Name
FCGR2B  [Similar Products]
UniProt Synonym Gene Names
CD32; FCG2; IGFR2; IgG Fc receptor II-b; Fc-gamma-RIIb; FcRII-b  [Similar Products]
UniProt Comments for CD32
Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.CautionHas sometimes been attributed to correspond to FcR-IIC.
Product References and Citations for anti-CD32 antibody
Stuart S.G., EMBO J. 8:3657-3666(1989). Brooks D.G., J. Exp. Med. 170:1369-1385(1989). Engelhardt W., Eur. J. Immunol. 20:1367-1377(1990).

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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