• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD34 recombinant protein

Scan QR to view Datasheet
Catalog # MBS553065
Unit / Price
  0.02 mg  /  $260 +1 FREE 8GB USB
  0.1 mg  /  $660 +1 FREE 8GB USB
  1 mg  /  $3,115 +3 FREE 8GB USB
CD34 recombinant protein
Product Name

CD34, Recombinant Protein

Full Product Name

Human CD34 Recombinant

Product Synonym Names
CD34; CD-34
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
142230
3D Structure
ModBase 3D Structure for P28906
Host
HEk293 Cells
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant CD34 is supplied as a 0.2 um filtered PBS solution, pH7.2.
Host Note
Optimized DNA sequence encoding Human extracellular domain of human CD34 including a C-terminal 6His tag was expressed in HEK293 cells.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Molecular Weight Note
Recombinant CD34 is a monomer protein consisting of 270 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of CD34 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CD34 recombinant protein
CD34 is a transmembrane glycoprotein expressed by human hematopoietic progenitor cells of lymphoid and myeloid lineage, and various types of colony-forming activity in human bone marrow (BM), cord blood (CB), and mobilized peripheral blood (PB) are contained in the CD34+ population. It has been suggested that CD34 also defines a normally occurring population of dendritic cells within the endoneurium of peripheral nerves that is distinct from fibroblasts and conventional Schwann cells
Product Categories/Family for CD34 recombinant protein
NCBI/Uniprot data below describe general gene information for CD34. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,716 Da
NCBI Official Full Name
hematopoietic progenitor cell antigen CD34 isoform a
UniProt Protein Name
Hematopoietic progenitor cell antigen CD34
UniProt Gene Name
UniProt Entry Name
CD34_HUMAN
NCBI Summary for CD34
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
UniProt Comments for CD34
CD34: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Belongs to the CD34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: intercellular bridge; integral to plasma membrane; lysosome; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; basal plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: sulfate binding; carbohydrate binding; transcription factor binding

Biological Process: positive regulation of odontogenesis; regulation of immune response; negative regulation of nitric oxide biosynthetic process; glomerular filtration; cell motility involved in cell locomotion; negative regulation of blood coagulation; negative regulation of tumor necrosis factor production; signal transduction; positive regulation of interleukin-10 production; cell proliferation; positive regulation of angiogenesis; cell-cell adhesion; endothelial cell proliferation; regulation of blood pressure; tissue homeostasis; hemopoiesis; leukocyte migration

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withCD34 recombinant protein
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000