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anti-CD4 antibody :: anti-Human CD4 Antibody

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Catalog # MBS859599
Unit / Price
  0.1 mg  /  $215 +1 FREE 8GB USB
Product Name

CD4, Antibody

Popular Item
Also Known As

CD4 (Phospho-Ser433) Antibody

Product Synonym Names
CD4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 precursor
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
458
OMIM
186940
3D Structure
ModBase 3D Structure for P01730
Species Reactivity
Human
Specificity
CD4 (Phospho-Ser433) antibody detects endogenous levels of CD4 only when phosphorylated at serine 433.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human CD4 around the phosphorylation site of serine 433 (R-M-SP-Q-I).
All Sites
Human: Ser433
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-CD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD4 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-CD4 antibody
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:40000

Testing Data of anti-CD4 antibody
P-peptide-+
anti-CD4 antibody Testing Data image
Immunohistochemistry (IHC) of anti-CD4 antibody
P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using CD4 (Phospho-Ser433) antibody. Immunofluorescence analysis of HepG2 cells, using CD4 (Phospho-Ser433) antibody.
anti-CD4 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CD4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
51,111 Da
NCBI Official Full Name
T-cell surface glycoprotein CD4 isoform 1
NCBI Official Synonym Full Names
CD4 molecule
NCBI Official Symbol
NCBI Official Synonym Symbols
CD4mut
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NCBI Protein Information
T-cell surface glycoprotein CD4
UniProt Protein Name
T-cell surface glycoprotein CD4
UniProt Synonym Protein Names
T-cell surface antigen T4/Leu-3; CD_antigen: CD4
Protein Family
UniProt Gene Name
NCBI Summary for CD4
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]
UniProt Comments for CD4
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
Product References and Citations for anti-CD4 antibody
GR Harriman, J. Immunol., Oct 1990; 145: 2406. G Zauli, Blood, Sep 1994; 84: 1896-1905. Andrew Zloza, Blood, Sep 2003; 102: 2156-2164. David M. Rothstein, Int. Immunol., Jan 1993; 5: 409-418.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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