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CD40LG elisa kit :: Pig CD40 ligand ELISA Kit

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Catalog # MBS2886234
Unit / Price
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  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $530 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,305 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,250 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CD40 ligand (CD40LG), ELISA Kit

Popular Item
Full Product Name

Pig CD40 ligand ELISA Kit

Product Synonym Names
CD40 ligand; CD40-L; Tumor necrosis factor ligand superfamily member 5; CD40LG; CD40L; TNFSF5; CD154
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2886234 Testing Data
3D Structure
ModBase 3D Structure for Q95MQ5
Species Reactivity
Specificity
Recombinant and natural Pig CD40 ligand,CD40-L
Samples
Serum, plasma, tissue homogenates,cell culture supernates or other biological fluids
Assay Type
Quantitative Competitive
Detection Range
0.312-20 ng/mL
Intra-assay Precision
Intra-Assay CV: <=5.3%
Inter-assay Precision
Inter-Assay CV: <=8.2%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CD40LG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CD40LGpurchase
MBS2886234 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD40 ligand (CD40LG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD40LG. The ELISA analytical biochemical technique of the MBS2886234 kit is based on CD40LG antibody-CD40LG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD40LG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD40LG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CD40LG elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates,cell culture supernates or other biological fluids.

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CD40LG elisa kit
CD40LG elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2886234. Click to request current manual
NCBI/Uniprot data below describe general gene information for CD40LG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
28,869 Da
NCBI Official Full Name
CD40 ligand
NCBI Official Symbol
CD40LG  [Similar Products]
NCBI Official Synonym Symbols
CD40L; TNFSF5
  [Similar Products]
NCBI Protein Information
CD40 ligand
UniProt Protein Name
CD40 ligand
UniProt Synonym Protein Names
Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154
Protein Family
UniProt Gene Name
CD40LG  [Similar Products]
UniProt Synonym Gene Names
CD40L; TNFSF5; CD40-L  [Similar Products]
UniProt Entry Name
CD40L_PIG
UniProt Comments for CD40LG
Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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