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CD5L elisa kit :: Human CD5L PicoKine ELISA Kit

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Catalog # MBS177570
Unit / Price
  96-Strip-Wells  /  $425 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CD5L PicoKine, ELISA Kit

Popular Item
Also Known As

Human CD5L PicoKine ELISA Kit

Product Synonym Names
CD5 antigen-like; AAC-11; AIM; API6; APOPTOSIS INHIBITOR 6; APOPTOSIS INHIBITOR OF MACROPHAGES; CD5 antigen like (scavenger receptor cysteine rich family); CD5 antigen-like; Cd5l; CD5L_HUMAN; CT 2; CT-2; Highly similar to ANTIGEN WC1.1 [Bos taurus]; IgM associated peptide; IgM-associated peptide; Pdp; PRO229; SCAVENGER RECEPTOR CYSTEINE-RICH FAMILY; SP ALPHA; SP-alpha; Spalpha; CD5 molecule-like
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
347
OMIM
602592
3D Structure
ModBase 3D Structure for O43866
Species Reactivity
Specificity
Natural and recombinant human CD5L
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Quantitative Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CD5L elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCD5Lpurchase
MBS177570 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD5L PicoKine, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD5L. The ELISA analytical biochemical technique of the MBS177570 kit is based on CD5L antibody-CD5L antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD5L antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD5L. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CD5L elisa kit
Principle of the Assay: The human CD5L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD5L has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: S20-G347) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD5L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD5L amount of sample captured in plate.

Background: CD5 antigen-like, also known as Sp alpha and AIM, is a protein that in humans is encoded by the CD5L gene. It is mapped to 1 q21-q23 by fluorescence in situ hybridization. It is found that Aim expression is induced in mouse macro phages in response to loading with highly oxidized low density lipoprotein (oxLDL), and that Aim is expressed in foam cells within atherosclerotic lesions. Both the expression of Aim in lesions and its induction by oxLDL require Lxr IRxr heterodimers. Aim-null macro phages are highly susceptible to oxLDL-induced apoptosis in vitro and undergo accelerated apoptosis in atherosclerotic lesions in vivo. Double knockout of Aim and Ldlr reduce atherosclerotic lesions. Therefore, it is concluded that AIM expression protects macrophages from apoptosis within atherosclerotic lesions, promoting early lesion development.

Typical Testing Data/Standard Curve (for reference only) of CD5L elisa kit
Human CD5L PicoKine ELISA Kit standard curve
CD5L elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS177570. Click to request current manual
NCBI/Uniprot data below describe general gene information for CD5L. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38,088 Da[Similar Products]
NCBI Official Full Name
CD5 antigen-like
NCBI Official Synonym Full Names
CD5 molecule like
NCBI Official Symbol
NCBI Official Synonym Symbols
AIM; API6; CT-2; hAIM; PRO229; Spalpha; SP-ALPHA
  [Similar Products]
NCBI Protein Information
CD5 antigen-like
UniProt Protein Name
CD5 antigen-like
UniProt Synonym Protein Names
Apoptosis inhibitor expressed by macrophages
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
API6; hAIM  [Similar Products]
UniProt Entry Name
CD5L_HUMAN
UniProt Comments for CD5L
CD5L: May play a role in the regulation of the immune system. Seems to play a role as an inhibitor of apoptosis.

Protein type: Secreted; Receptor, misc.; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cytoplasm; extracellular region; extracellular space; membrane

Molecular Function: scavenger receptor activity

Biological Process: apoptosis; cellular defense response; immune system process; inflammatory response; receptor-mediated endocytosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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