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CD80 elisa kit :: Human T-lymphocyte activation antigen CD80 ELISA Kit

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Catalog # MBS760940
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Product Name

T-lymphocyte activation antigen CD80, ELISA Kit

Also Known As

Human T-lymphocyte activation antigen CD80 ELISA Kit

Product Synonym Names
CD80/B7-1/T-lymphocyte activation antigen CD80
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
288
OMIM
112203
Species Reactivity
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
78-5000pg/ml
Sensitivity
46.9pg/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CD80 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCD80purchase
MBS760940 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the T-lymphocyte activation antigen CD80, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD80. The ELISA analytical biochemical technique of the MBS760940 kit is based on CD80 antibody-CD80 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD80 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD80. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CD80 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human CD80 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CD80 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD80 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CD80 can be calculated.

Typical Testing Data of CD80 elisa kit
CD80 elisa kit Typical Testing Data image
Typical Standard Curve of CD80 elisa kit
CD80 elisa kit Typical Standard Curve image
Sample Manual Insert of MBS760940. Click to request current manual
NCBI/Uniprot data below describe general gene information for CD80. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18,761 Da
NCBI Official Full Name
T-lymphocyte activation antigen CD80
NCBI Official Synonym Full Names
CD80 molecule
NCBI Official Symbol
NCBI Official Synonym Symbols
B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
  [Similar Products]
NCBI Protein Information
T-lymphocyte activation antigen CD80
UniProt Protein Name
T-lymphocyte activation antigen CD80
UniProt Synonym Protein Names
Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD_antigen: CD80
UniProt Gene Name
UniProt Synonym Gene Names
CD28LG; CD28LG1; LAB7; B7  [Similar Products]
UniProt Entry Name
CD80_HUMAN
NCBI Summary for CD80
The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]
UniProt Comments for CD80
CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.3-q21

Cellular Component: cell surface; external side of plasma membrane; integral to membrane; intracellular; plasma membrane

Molecular Function: coreceptor activity; protein binding; viral receptor activity

Biological Process: cell-cell signaling; entry of virus into host cell; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of alpha-beta T cell proliferation; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of interleukin-2 biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of signal transduction; positive regulation of T-helper 1 cell differentiation; positive regulation of transcription, DNA-dependent; T cell activation; T cell costimulation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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