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CD80 elisa kit :: Mouse B7-1/CD80 ELISA Kit

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Catalog # MBS175825
Unit / Price
  96-Strip-Wells  /  $455 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

B7-1/CD80, ELISA Kit

Popular Item
Also Known As

Mouse B7-1/CD80 PicoKine ELISA Kit

Product Synonym Names
T-lymphocyte activation antigen CD80; CD80 molecule; Activation B7-1 antigen; B7; BB1; CD80; CD80_HUMAN; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
306
3D Structure
ModBase 3D Structure for Q00609
Species Reactivity
Specificity
Natural and recombinant mouse CD80
Samples
Cell culture supernates and serum
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CD80 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCD80purchase
MBS175825 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the B7-1/CD80, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD80. The ELISA analytical biochemical technique of the MBS175825 kit is based on CD80 antibody-CD80 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD80 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD80. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CD80 elisa kit
Principle of the assay: mouse CD80 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD80 has been precoated onto 96-well plates. Standards(NSO, D37-K245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD80 amount of sample captured in plate.
Background: The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain[1].The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.[2] stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1,D37—K245,which is composed of two single chains acids with the dipolymer.

Typical Testing Data/Standard Curve (for reference only) of CD80 elisa kit
Mouse B7-1/CD80 PicoKine ELISA Kit standard curve
CD80 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS175825. Click to request current manual
NCBI/Uniprot data below describe general gene information for CD80. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,051 Da
NCBI Official Full Name
T-lymphocyte activation antigen CD80
NCBI Official Synonym Full Names
CD80 antigen
NCBI Official Symbol
NCBI Official Synonym Symbols
B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
  [Similar Products]
NCBI Protein Information
T-lymphocyte activation antigen CD80; B7 protein; activation B7-1 antigen
UniProt Protein Name
T-lymphocyte activation antigen CD80
UniProt Synonym Protein Names
Activation B7-1 antigen; B7; CD_antigen: CD80
UniProt Gene Name
UniProt Synonym Gene Names
B7; B7  [Similar Products]
UniProt Entry Name
CD80_MOUSE
UniProt Comments for CD80
CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; T cell costimulation; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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