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CD97 elisa kit :: Mouse CD97 ELISA Kit

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Catalog # MBS2507901
Unit / Price
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  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CD97, ELISA Kit

Popular Item
Full Product Name

Mouse CD97 (Cluster of Differentiation 97) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
601211
3D Structure
ModBase 3D Structure for P48960
Species Reactivity
Specificity
This kit recognizes natural and recombinant Mouse CD97. No significant cross-reactivity or interference between Mouse CD97 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.781-50ng/mL
Sensitivity
Min: 0.469ng/mL; Max: 50ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CD97 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CD97purchase
MBS2507901 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD97, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD97. The ELISA analytical biochemical technique of the MBS2507901 kit is based on CD97 antibody-CD97 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD97 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD97. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CD97 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse CD97 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD97. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD97 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD97, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CD97. You can calculate the concentration of CD97 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CD97 elisa kit
CD97 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2507901. Click to request current manual
NCBI/Uniprot data below describe general gene information for CD97. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
86,628 Da
NCBI Official Full Name
CD97 antigen isoform 1 preproprotein
NCBI Official Synonym Full Names
CD97 molecule
NCBI Official Symbol
NCBI Official Synonym Symbols
TM7LN1
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NCBI Protein Information
CD97 antigen; leukocyte antigen CD97; seven transmembrane helix receptor; seven-span transmembrane protein; seven-transmembrane, heterodimeric receptor associated with inflammation
UniProt Protein Name
CD97 antigen
UniProt Synonym Protein Names
Leukocyte antigen CD97; CD_antigen: CD97Cleaved into the following 2 chains:CD97 antigen subunit alpha; CD97 antigen subunit beta
Protein Family
UniProt Gene Name
UniProt Entry Name
CD97_HUMAN
NCBI Summary for CD97
This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]
UniProt Comments for CD97
CD97: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 2; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: extracellular space; focal adhesion; membrane; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; transmembrane receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; cell-cell signaling; immune response; cell adhesion; cell motility; inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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