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anti-CDC25A antibody :: Rabbit CDC25A-S76 Antibody

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Catalog # MBS128525
Unit / Price
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  0.05 mL  /  $235 +1 FREE 8GB USB
  0.1 mL  /  $350 +1 FREE 8GB USB
  0.2 mL  /  $525 +1 FREE 8GB USB
Product Name

CDC25A-S76, Antibody

Popular Item
Full Product Name

phospho-CDC25A-S76 Polyclonal Antibody

Product Synonym Names
Phospho-CDC25A-S76: CDC25A2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
116947
3D Structure
ModBase 3D Structure for P30304
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Route
Synthetic Peptide
Immunogen
A phospho specific peptide corresponding to residues surrounding S76 of human CDC25A
Calculated Molecular Weight
70kDa
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-CDC25A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CDC25A antibody
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-CDC25A antibody
Applications Tested/Suitable for anti-CDC25A antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CDC25A antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100

Western Blot (WB) of anti-CDC25A antibody
Western blot analysis of extracts from HeLa cells using phospho-CDC25A-S76 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
anti-CDC25A antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CDC25A antibody
Immunohistochemistry of paraffin-embedded human breast carcinoma using phospho-CDC25A-S76 antibody.
anti-CDC25A antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CDC25A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
M-phase inducer phosphatase 1
NCBI Official Synonym Full Names
cell division cycle 25A
NCBI Official Symbol
CDC25A  [Similar Products]
NCBI Official Synonym Symbols
CDC25A2
  [Similar Products]
NCBI Protein Information
M-phase inducer phosphatase 1; CDC25A2-CAG isoform; CDC25 isoform A1-CAG; dual specificity phosphatase CDC25A
UniProt Protein Name
M-phase inducer phosphatase 1
UniProt Synonym Protein Names
Dual specificity phosphatase Cdc25A
UniProt Gene Name
CDC25A  [Similar Products]
UniProt Entry Name
MPIP1_HUMAN
NCBI Summary for CDC25A
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for CDC25A
CDC25A: a member of the MPI phosphatase family. Functions as a dosage-dependent inducer in mitotic control. Required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two splice variant isoforms have been described.

Protein type: Protein phosphatase, dual-specificity; EC 3.1.3.48; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: cell proliferation; mitosis; response to radiation; cell division; regulation of cell cycle; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; DNA replication; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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