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CDC25B elisa kit :: Human cell division cycle 25 homolog B (S. pombe) ELISA Kit

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Catalog # MBS931754
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Typical Testing Data/Standard Curve (for reference only)
Product Name

cell division cycle 25 homolog B (S. pombe) (CDC25B), ELISA Kit

Popular Item
Full Product Name

Human M-phase inducer phosphatase 2, CDC25B ELISA Kit

Product Synonym Names
Human M-phase inducer phosphatase 2 (CDC25B) ELISA kit; RP5-1009E24.3; cell division cycle 25B; cell division cycle 25 homolog B (S. pombe)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS931754 Testing Data
OMIM
116949
3D Structure
ModBase 3D Structure for P30305
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human CDC25B. No significant cross-reactivity or interference between Human CDC25B and analogues was observed.
Samples
Serum, plasma, tissue homogenates and Cell lysates
Assay Type
Sandwich
Detection Range
39.06 pg/ml-2500 pg/ml
Sensitivity
9.75 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Cell Cycle
Protein Biological Process 3
Cell cycle
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CDC25B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCDC25Bpurchase
MBS931754 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cell division cycle 25 homolog B (S. pombe) (CDC25B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CDC25B. The ELISA analytical biochemical technique of the MBS931754 kit is based on CDC25B antibody-CDC25B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDC25B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDC25B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CDC25B elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CDC25B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CDC25B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CDC25B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CDC25B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CDC25B elisa kit
CDC25B elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS931754. Click to request current manual
NCBI/Uniprot data below describe general gene information for CDC25B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
64,987 Da
NCBI Official Full Name
M-phase inducer phosphatase 2 isoform 7
NCBI Official Synonym Full Names
cell division cycle 25B
NCBI Official Symbol
CDC25B  [Similar Products]
NCBI Protein Information
M-phase inducer phosphatase 2; dual specificity phosphatase Cdc25B
UniProt Protein Name
M-phase inducer phosphatase 2
UniProt Synonym Protein Names
Dual specificity phosphatase Cdc25B
UniProt Gene Name
CDC25B  [Similar Products]
UniProt Synonym Gene Names
CDC25HU2  [Similar Products]
UniProt Entry Name
MPIP2_HUMAN
NCBI Summary for CDC25B
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]
UniProt Comments for CDC25B
CDC25B: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, dual-specificity; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: nucleoplasm; spindle pole; centrosome; cytoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: positive regulation of cytokinesis; mitosis; cell division; positive regulation of protein kinase activity; positive regulation of cell proliferation; mitotic cell cycle; positive regulation of mitotic cell cycle; G2/M transition of mitotic cell cycle; protein amino acid phosphorylation; oocyte maturation; female meiosis I
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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