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CDC7/DBF4 recombinant protein

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Catalog # MBS515469
Unit / Price
  0.005 mg  /  $315 +1 FREE 8GB USB
  0.01 mg  /  $425 +1 FREE 8GB USB
Product Name

CDC7/DBF4, Recombinant Protein

Popular Item
Also Known As

CDC7/DBF4, Active

Product Synonym Gene Name
CDC7: CDC7L1; HsCDC7; Hsk1; huCDC7; MGC117361; MGC126237; MGC126238[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
Full Length
Sequence Length
3222
OMIM
AB003698 mRNA
3D Structure
ModBase 3D Structure for O00311
Host
Sf9 insect cells using baculovirus
Purity/Purification
>85%
Form/Format
Recombinant protein stored in 50mM Tris-HCI,pH 7.5, 150mM NaCI, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1 mM PMSF, 25% Glycerol.
Concentration
0.1 ug/uL (lot specific)
Specific activity
8.5 nmol/min/mg
Preparation and Storage
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Shipping: Ships in dry ice.

Stability:1 year at -70 from date of shipment.
Other Notes
Small volumes of CDC7/DBF4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CDC7/DBF4 recombinant protein
Description: Recombinant full-length human CDC7 and DBF4 proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tags.

Scientific Background: CDC7 is a cell division cycle 7 homolog protein that is critical for the G1/S transition and is also essential for initiation of DNA replication as cell division occurs. CDC7 is expressed in many normal tissues, but the overexpression of CDC7 may be associated with neoplastic transformation for some tumors and transformed cell lines (1). CDC7/DBF4 kinase promotes S phase by alleviating an inhibitory activity in Mcm4 that evolved to integrate several protein kinase (2).
Applications Tested/Suitable for CDC7/DBF4 recombinant protein
Kinase Assay, Western Blot (WB)

CDC7/DBF4 recombinant protein Testing Data TD image
CDC7/DBF4 recombinant protein Testing Data TD image
NCBI/Uniprot data below describe general gene information for CDC7/DBF4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens cell division cycle 7 (CDC7), transcript variant 1, mRNA
NCBI Official Synonym Full Names
cell division cycle 7
NCBI Official Symbol
NCBI Official Synonym Symbols
Hsk1; CDC7L1; HsCDC7; huCDC7
  [Similar Products]
NCBI Protein Information
cell division cycle 7-related protein kinase
UniProt Protein Name
Cell division cycle 7-related protein kinase
UniProt Gene Name
UniProt Synonym Gene Names
CDC7L1; CDC7-related kinase; HsCdc7; huCdc7  [Similar Products]
NCBI Summary for CDC7/DBF4
This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq, Aug 2008]
UniProt Comments for CDC7/DBF4
CDC7: an evolutionarily conserved serine/threonine protein kinase of the CDC7 family. Predominantly localized in the nucleus, it plays a pivotal role in linking cell cycle regulation to genome duplication, being essential for the firing of DNA replication origins. Plays an essential role in initiating DNA replication at replication origins by phosphorylating the MCM2 and MCM4 subunits of the MCM2-7 helicase complex. The human protein requires phosphorylation by CDK1 at T376 and binding to activator of S-phase kinase (ASK)/DBF4B to be fully activated. The interaction between Cdc7 and DBFK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase. Although CDC7 expression levels appear to be constant throughout the cell cycle, the expression levels of DBF4B is cell cycle-regulated. Thus, the CDC7 activity is activated during S phase in eukaryotes. The Cdc7/ DBF4B complex regulates initiation of DNA replication by phosphorylating chromatin-bound mini-chromosome maintenance protein 2 (MCM2). Impaired Cdc7 kinase activity leads to S-phase arrest. Plays a role in the TGF-beta regulation of smooth muscle cell (SMC) differentiation. Interacts with Smad3 to facilitate Smad3 binding to SMC marker promoter, leading to the activation of SMC marker gene transcription.

Protein type: CDC7 family; EC 2.7.11.1; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: cytoplasm; intercellular bridge; nucleoplasm; nucleus

Molecular Function: kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: DNA replication; DNA replication initiation; double-strand break repair via break-induced replication; G1/S transition of mitotic cell cycle; peptidyl-serine phosphorylation; phagocytosis; positive regulation of cell proliferation; regulation of cell shape
Product References and Citations for CDC7/DBF4 recombinant protein
1. Hess, G. F. et.al: A human homolog of the yeast CDC7 gene is overexpressed in some tumors and transformed cell lines. Gene 211: 133-140, 1998. 2. Sheu, Y.-J. et.al: The Dbf4-Cdc7 kinase promotes S phase by alleviating an inhibitory activity in Mcm4. Nature 463: 113-117, 2010.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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