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CDCP1 elisa kit :: Mouse CUB Domain Containing Protein 1, CDCP1 ELISA Kit

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Catalog # MBS7232456
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

CUB Domain Containing Protein 1, CDCP1, ELISA Kit

Popular Item
Also Known As

Mouse CUB Domain Containing Protein 1, CDCP1 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
611735
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of CDCP1. No significant cross-reactivity or interference between CDCP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDCP1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CDCP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCDCP1purchase
MBS7232456 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CUB Domain Containing Protein 1, CDCP1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CDCP1. The ELISA analytical biochemical technique of the MBS7232456 kit is based on CDCP1 antibody-CDCP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDCP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDCP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CDCP1 elisa kit
Intended Uses: This CDCP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CDCP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CDCP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDCP1 antibody and an CDCP1-HRP conjugate. The assay sample and buffer are incubated together with CDCP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDCP1 concentration since CDCP1 from samples and CDCP1-HRP conjugate compete for the anti-CDCP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDCP1 from the sample, fewer sites are left to bind CDCP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDCP1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CDCP1 elisa kit

Typical Standard Curve/Testing Data of CDCP1 elisa kit
CDCP1 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS7232456. Click to request current manual
NCBI/Uniprot data below describe general gene information for CDCP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
37,818 Da
NCBI Official Full Name
CUB domain-containing protein 1 isoform 1
NCBI Official Synonym Full Names
CUB domain containing protein 1
NCBI Official Symbol
CDCP1  [Similar Products]
NCBI Official Synonym Symbols
CD318; TRASK; SIMA135
  [Similar Products]
NCBI Protein Information
CUB domain-containing protein 1; membrane glycoprotein gp140; transmembrane and associated with src kinases; subtractive immunization M plus HEp3-associated 135 kDa protein
UniProt Protein Name
CUB domain-containing protein 1
UniProt Synonym Protein Names
Membrane glycoprotein gp140; Subtractive immunization M plus HEp3-associated 135 kDa protein; SIMA135; Transmembrane and associated with src kinases; CD_antigen: CD318
UniProt Gene Name
CDCP1  [Similar Products]
UniProt Synonym Gene Names
TRASK; UNQ2486/PRO5773; SIMA135  [Similar Products]
UniProt Entry Name
CDCP1_HUMAN
NCBI Summary for CDCP1
This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
UniProt Comments for CDCP1
CDCP1: a transmembrane protein containing three extracellular CUB domains involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation depending upon its phosphorylation status. Interacts with N- and P-cadherin, syndecans 1 and 4, the serine protease ST14, SRC and PKCG. Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Its expression level is correlated with the metastatic ability of carcinoma cells. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. Three alternatively spliced human isoforms have been reported. Isoform 1 is a single-pass membrane protein that may also be shed, giving rise to a soluble peptide. Isoform 3 is secreted.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: plasma membrane; extracellular region; integral to membrane
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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