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CDH5 elisa kit :: Rat Cadherin 5 (CDH5) ELISA Kit

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Catalog # MBS2019699 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $270 +1 FREE 8GB USB
  48-Strip-Wells  /  $505 +1 FREE 8GB USB
  96-Strip-Wells  /  $685 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,775 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,035 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Cadherin 5 (CDH5), ELISA Kit

Popular Item
Also Known As

Cadherin 5 (CDH5) ELISA Kit

Product Synonym Names
CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
784
3D Structure
ModBase 3D Structure for P55284
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Cadherin 5 (CDH5).
No significant cross-reactivity or interference between Cadherin 5 (CDH5) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
78-5000pg/mL
Sensitivity
< 27pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Cadherin 5 (CDH5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Cadherin 5 (CDH5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CDH5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCDH5purchase
MBS2019699 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cadherin 5 (CDH5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CDH5. The ELISA analytical biochemical technique of the MBS2019699 kit is based on CDH5 antibody-CDH5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDH5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDH5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CDH5 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin 5 (CDH5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin 5 (CDH5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin 5 (CDH5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cadherin 5 (CDH5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CDH5 elisa kit
CDH5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2019699. Click to request current manual
NCBI/Uniprot data below describe general gene information for CDH5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
87,903 Da
NCBI Official Full Name
Cadherin 5
NCBI Official Synonym Full Names
cadherin 5
NCBI Official Symbol
NCBI Official Synonym Symbols
7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225
  [Similar Products]
NCBI Protein Information
cadherin-5
UniProt Protein Name
Cadherin-5
UniProt Synonym Protein Names
Vascular endothelial cadherin; VE-cadherin; CD_antigen: CD144
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
VE-cadherin  [Similar Products]
UniProt Entry Name
CADH5_MOUSE
UniProt Comments for CDH5
CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Cellular Component: tight junction; adherens junction; membrane; integral to membrane; plasma membrane; intercellular junction; cell junction

Molecular Function: protein binding; metal ion binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding

Biological Process: negative regulation of cell proliferation; cell-cell adhesion; homophilic cell adhesion; cell adhesion; blood vessel maturation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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