• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CDK-9 elisa kit :: Human CDK9 ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2502653
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CDK9 (CDK-9), ELISA Kit

Popular Item
Full Product Name

Human CDK9 (Cyclin Dependent Kinase 9) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2502653 COA
OMIM
603251
3D Structure
ModBase 3D Structure for P50750
Species Reactivity
Specificity
This kit recognizes natural and some recombinant Human CDK9. No significant crossreactivity or interference between Human CDK9 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
1.563-100ng/mL
Sensitivity
Min: 0.938ng/mL; Max: 100ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CDK-9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCDK-9purchase
MBS2502653 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CDK9 (CDK-9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CDK-9. The ELISA analytical biochemical technique of the MBS2502653 kit is based on CDK-9 antibody-CDK-9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDK-9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDK-9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CDK-9 elisa kit
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human CDK9 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human CDK9. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human CDK9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human CDK9, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CDK9. The concentration of Human CDK9 in samples can be calculated by comparing the OD of the samples with the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CDK-9 elisa kit
CDK-9 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2502653. Click to request current manual
NCBI/Uniprot data below describe general gene information for CDK-9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
53,365 Da
NCBI Official Full Name
cyclin-dependent kinase 9
NCBI Official Synonym Full Names
cyclin-dependent kinase 9
NCBI Official Symbol
NCBI Official Synonym Symbols
TAK; C-2k; CTK1; CDC2L4; PITALRE
  [Similar Products]
NCBI Protein Information
cyclin-dependent kinase 9; CDC2-related kinase; cell division cycle 2-like protein kinase 4; cell division protein kinase 9; serine/threonine protein kinase PITALRE; tat-associated kinase complex catalytic subunit
UniProt Protein Name
Cyclin-dependent kinase 9
UniProt Synonym Protein Names
C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit
UniProt Gene Name
UniProt Synonym Gene Names
CDC2L4; TAK  [Similar Products]
UniProt Entry Name
CDK9_HUMAN
NCBI Summary for CDK-9
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]
UniProt Comments for CDK-9
CDK9: a protein kinase of the CDK family. Forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. A component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. Transcriptional elongation factor and cofactor for HIV Tat protein; RNAi blocks HIV replication, and inhibitors also block varicella zoster replication. Mediates signals leading to cardiac hypertrophy. Inhibitor: Flavopiridol.

Protein type: Cell cycle regulation; EC 2.7.11.23; Nuclear receptor co-regulator; Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.22; CMGC group; CDK family; CDK/CDK9 subfamily; CDK9 subfamily

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; transcription elongation factor complex; PML body; transcription elongation factor complex b; intracellular membrane-bound organelle; membrane; cytoplasm

Molecular Function: RNA polymerase subunit kinase activity; protein binding; snRNA binding; DNA binding; cyclin-dependent protein kinase activity; chromatin binding; ATP binding; protein kinase activity

Biological Process: regulation of histone modification; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; transcription, DNA-dependent; positive regulation of histone phosphorylation; positive regulation of viral transcription; regulation of mitotic cell cycle; DNA repair; protein amino acid phosphorylation; regulation of DNA repair; cell proliferation; transforming growth factor beta receptor signaling pathway; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; replication fork arrest
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000