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CDK4 elisa kit :: Rat Cyclin Dependent Kinase 4 ELISA Kit

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Catalog # MBS2533543
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Cyclin Dependent Kinase 4 (CDK4), ELISA Kit

Popular Item
Full Product Name

Rat CDK4 (Cyclin Dependent Kinase 4) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
123829
3D Structure
ModBase 3D Structure for P11802
Species Reactivity
Specificity
This kit recognizes natural and recombinantRatCDK4. No significant cross-reactivity or interference between RatCDK4 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
78.125-5000pg/mL
Sensitivity
Min: 46.875pg/mL ; Max: 5000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CDK4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCDK4purchase
MBS2533543 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cyclin Dependent Kinase 4 (CDK4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CDK4. The ELISA analytical biochemical technique of the MBS2533543 kit is based on CDK4 antibody-CDK4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDK4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDK4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CDK4 elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatCDK4concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CDK4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CDK4and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CDK4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CDK4.You can calculate the concentration of CDK4in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CDK4 elisa kit
CDK4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2533543. Click to request current manual
NCBI/Uniprot data below describe general gene information for CDK4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,725 Da
NCBI Official Full Name
cyclin-dependent kinase 4
NCBI Official Synonym Full Names
cyclin dependent kinase 4
NCBI Official Symbol
NCBI Official Synonym Symbols
CMM3; PSK-J3
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NCBI Protein Information
cyclin-dependent kinase 4
UniProt Protein Name
Cyclin-dependent kinase 4
UniProt Synonym Protein Names
Cell division protein kinase 4; PSK-J3
Protein Family
UniProt Gene Name
UniProt Entry Name
CDK4_HUMAN
NCBI Summary for CDK4
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008]
UniProt Comments for CDK4
CDK4: a protein kinase of the CDK family that is important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. Controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). Phosphorylates the retinoblastoma gene product (Rb). Point mutations found in somatic and familial melanoma. Amplified in sarcomas, glioma and lymphoma. Amplified, methylated or deleted in head and neck squamous cell carcinoma. Overexpression drives epithelial tumors in mice. Disruption makes mice resistant to cancer. Inhibitor: PD332991.

Protein type: Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; EC 2.7.11.22; Protein kinase, CMGC; Kinase, protein; CMGC group; CDK family; CDK/CDK4 subfamily; CDK4 subfamily

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: chromatin; cyclin-dependent protein kinase holoenzyme complex; cytosol; nuclear membrane; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm; tight junction; transcription factor complex

Molecular Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; cyclin-dependent protein kinase regulator activity; protein binding; protein complex binding

Biological Process: cell division; circadian rhythm; establishment and/or maintenance of chromatin architecture; G1/S transition of mitotic cell cycle; lens development in camera-type eye; mitotic cell cycle; organ regeneration; positive regulation of apoptosis; positive regulation of cell proliferation; positive regulation of cell size; positive regulation of fibroblast proliferation; positive regulation of translation; protein amino acid phosphorylation; regulation of gene expression; regulation of protein kinase activity; response to drug; response to hyperoxia; response to lead ion; response to testosterone stimulus; response to toxin; signal transduction

Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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