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CDK5P25 recombinant protein

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Catalog # MBS515301
Unit / Price
  0.02 mg  /  $260 +1 FREE 8GB USB
  0.05 mg  /  $415 +1 FREE 8GB USB
SDS-PAGE
Product Name

CDK5P25, Recombinant Protein

Popular Item
Also Known As

CDK5P25 recombinant protein

Product Synonym Names
p25; CDK5R1; CDK5R; NCK5A; p23; CDK5P25 Protein
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
3870
Chromosome Location
Chromosome: 17; NC_000017.10 (30814105..30818271). Location: 17q11.2
OMIM
603460
3D Structure
ModBase 3D Structure for Q15078
Host
Sf9 insect cells
Purity/Purification
>85%
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.2 ug/ul (lot specific)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
N-terminal GST
Source Note
Recombinant human CDK5R1 (98-end) was expressed by baculovirus in Sf9 insect cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Stable for 1 year at -70 degree C from date of shipment.
Other Notes
Small volumes of CDK5P25 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for CDK5P25 recombinant protein
Applications Tested/Suitable for CDK5P25 recombinant protein
Western Blot (WB)

The purity of CDK5P25 was determined to be >85% by densitometry. Approx. MW 49kDa.
CDK5P25 recombinant protein SDS-PAGE image
NCBI/Uniprot data below describe general gene information for CDK5P25. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
49 kDa
NCBI Official Full Name
Homo sapiens cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1), mRNA
NCBI Official Synonym Full Names
cyclin-dependent kinase 5, regulatory subunit 1 (p35)
NCBI Official Symbol
CDK5R1  [Similar Products]
NCBI Official Synonym Symbols
p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
  [Similar Products]
NCBI Protein Information
cyclin-dependent kinase 5 activator 1; CDK5 activator 1; neuronal CDK5 activator; TPKII regulatory subunit; regulatory partner for CDK5 kinase; tau protein kinase II 23kDa subunit; cyclin-dependent kinase 5 regulatory subunit 1
UniProt Protein Name
Cyclin-dependent kinase 5 activator 1
UniProt Synonym Protein Names
Cyclin-dependent kinase 5 regulatory subunit 1; TPKII regulatory subunitCleaved into the following 2 chains:Cyclin-dependent kinase 5 activator 1, p35; p35Cyclin-dependent kinase 5 activator 1, p25; p25; Alternative name(s):; Tau protein kinase II 23 kDa subunit
UniProt Gene Name
CDK5R1  [Similar Products]
UniProt Synonym Gene Names
CDK5R; NCK5A; CDK5 activator 1; p35; p25; p23  [Similar Products]
UniProt Entry Name
CD5R1_HUMAN
NCBI Summary for CDK5P25
The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008]
UniProt Comments for CDK5P25
Function: p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII.

Subunit structure: Heterodimer composed of a catalytic subunit CDK5 and a regulatory subunit CDK5R1 (p25) and macromolecular complex composed of at least CDK5, CDK5R1 (p35) and CDK5RAP1 or CDK5RAP2 or CDK5RAP3. Only the heterodimer shows kinase activity. Interacts with EPHA4 and NGEF; may mediate the activation of NGEF by EPHA4

By similarity. Interacts with RASGRF2. Ref.8 Ref.10

Subcellular location: Cyclin-dependent kinase 5 activator 1, p35: Cell membrane; Lipid-anchor; Cytoplasmic side

Probable. Note: In the primary cortical neurons, p35 is present in the peripheries and nerve terminals. Ref.6 Ref.10 Ref.11Cyclin-dependent kinase 5 activator 1, p25: Nucleus. Cytoplasm › perinuclear region. Note: The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites. Ref.6 Ref.10 Ref.11

Tissue specificity: Brain and neuron specific.

Post-translational modification: The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo

By similarity.Myristoylated. A proper myristoylation signal is essential for the proper distribution of p35.Ubiquitinated. Degradation of p35 by proteasome results in down-regulation of CDK5 activity. During this process, CDK5 phosphorylates p35 and induces its ubiquitination and subsequent degradation. Ref.7Phosphorylation at Ser-8 and Thr-138 by CDK5 prevents calpain-mediated proteolysis.

Miscellaneous: Cleavage of p35 to p25 may be involved in the pathogenesis of cytoskeletal abnormalities and neuronal death in neurodegenerative diseases. The p25 form accumulates in neurons in the brain of patients with Alzheimer disease, but not in normal brain. This accumulation correlates with an increase in CDK5 kinase activity. Application of amyloid beta peptide A-beta(1-42) induced the conversion of p35 to p25 in primary cortical neurons. Expression of the p25/Cdk5 complex in cultured primary neurons induces cytoskeletal disruption, morphological degeneration and apoptosis.

Sequence similarities: Belongs to the cyclin-dependent kinase 5 activator family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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