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CDK8 recombinant protein

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Catalog # MBS515108
Unit / Price
  0.02 mg  /  $270 +1 FREE 8GB USB
  0.05 mg  /  $450 +1 FREE 8GB USB
SDS-PAGE
Product Name

CDK8, Recombinant Protein

Popular Item
Full Product Name

CDK8, Unactive recombinant protein

Product Synonym Names
K35; MGC126074; MGC126075; CDK8; Unacitve
Product Synonym Gene Name
K35; MGC126074; MGC126075[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 13; NC_000013.10 (26828262..26979375). Location: 13q12
OMIM
603184
3D Structure
ModBase 3D Structure for P49336
Host
Sf9 insect cells
Purity/Purification
>80%
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Concentration
0.2ug/ul (lot specific)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human CDK8 was expressed by baculovirus in Sf9 insect cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: Ships with Dry Ice.
Other Notes
Small volumes of CDK8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CDK8 recombinant protein
Recombinant full-length human CDK8 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: CDK8 is a member of the CDK family which are important regulators of cell cycle progression. CDK8 associates with Cyclin C and is a component of the RNA polymerase II holoenzyme complex that phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (1). CDK8 regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH). Inhibition of CDK8 expression inhibits proliferation in colon cancer cells characterized by high levels of CDK8 and beta-catenin hyperactivity. CDK8 kinase activity is necessary for beta-catenin-driven transformation and for expression of several beta-catenin transcriptional targets (2).
Applications Tested/Suitable for CDK8 recombinant protein
Kinase Assay, Western Blot (WB)

NCBI/Uniprot data below describe general gene information for CDK8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
~84 kDa
NCBI Official Full Name
Homo sapiens cyclin-dependent kinase 8, mRNA
NCBI Official Synonym Full Names
cyclin-dependent kinase 8
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
cyclin-dependent kinase 8; protein kinase K35; CDK8 protein kinase; mediator complex subunit CDK8; cell division protein kinase 8; mediator of RNA polymerase II transcription subunit CDK8
UniProt Protein Name
Cyclin-dependent kinase 8
UniProt Synonym Protein Names
Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35
Protein Family
UniProt Gene Name
UniProt Entry Name
CDK8_HUMAN
NCBI Summary for CDK8
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery. [provided by RefSeq, Jul 2008]
UniProt Comments for CDK8
Function: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. Ref.8 Ref.9

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Ref.8ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate. Ref.8

Cofactor: Magnesium

By similarity.

Subunit structure: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. The cylin/CDK pair formed by CCNC/CDK8 also associates with the large subunit of RNA polymerase II. Interacts with CTNNB1, GLI3 and MAML1. Ref.5 Ref.6 Ref.9 Ref.10 Ref.11 Ref.12

Subcellular location: Nucleus

Probable.

Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Contains 1 protein kinase domain.
Product References and Citations for CDK8 recombinant protein
1.Akoulitchev S, et al: TFIIH is negatively regulated by cdk8-containing mediator complexes. Nature 407: 102-106, 2000.
2.Firestein R, et al: CDK8 is a colorectal cancer oncogene that regulates beta-catenin activity. Nature 455: 547-551, 2008.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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