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CEBPb elisa kit :: Human CCAAT/Enhancer Binding Protein Beta (CEBPb) ELISA Kit

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Catalog # MBS2702338 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

CCAAT/Enhancer Binding Protein Beta (CEBPb), ELISA Kit

Full Product Name

Human CCAAT/Enhancer Binding Protein Beta (CEBPb) ELISA Kit

Product Synonym Names
CRP2; IL6DBP; LAP; NF-IL6; TCF5; C/EBP-Beta; Liver-Enriched Transcriptional Activator Protein; Nuclear Factor Of Interleukin 6; Interleukin 6-Dependent DNA-Binding Protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P28033
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of CCAAT/Enhancer Binding Protein Beta (CEBPb).
No significant cross-reactivity or interference between CCAAT/Enhancer Binding Protein Beta (CEBPb) and analogues was observed.
Assay Type
Double-antibody Sandwich
Tissue Homogenates, Cell Lysates and Other Biological Fluids
Detection Range
< 0.061ng/mL
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level CCAAT/Enhancer Binding Protein Beta (CEBPb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level CCAAT/Enhancer Binding Protein Beta (CEBPb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CEBPb elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CEBPbpurchase
MBS2702338 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CCAAT/Enhancer Binding Protein Beta (CEBPb) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CEBPb. The ELISA analytical biochemical technique of the MBS2702338 kit is based on CEBPb antibody-CEBPb antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CEBPb antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CEBPb. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CEBPb elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCAAT/Enhancer Binding Protein Beta (CEBPb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CCAAT/Enhancer Binding Protein Beta (CEBPb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CCAAT/Enhancer Binding Protein Beta (CEBPb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CCAAT/Enhancer Binding Protein Beta (CEBPb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CEBPb elisa kit
CEBPb elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2702338. Click to request current manual
NCBI/Uniprot data below describe general gene information for CEBPb. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
15,597 Da
NCBI Official Full Name
CCAAT/enhancer-binding protein beta isoform b
NCBI Official Synonym Full Names
CCAAT/enhancer binding protein (C/EBP), beta
NCBI Official Symbol
Cebpb  [Similar Products]
NCBI Official Synonym Symbols
LAP; LIP; CRP2; NF-M; Nfil6; NF-IL6; IL-6DBP; C/EBPbeta
  [Similar Products]
NCBI Protein Information
CCAAT/enhancer-binding protein beta
UniProt Protein Name
CCAAT/enhancer-binding protein beta
UniProt Synonym Protein Names
AGP/EBP; Interleukin-6-dependent-binding protein; IL-6DBP; Liver-enriched transcriptional activator; LAP
UniProt Gene Name
Cebpb  [Similar Products]
UniProt Synonym Gene Names
C/EBP beta; IL-6DBP; LAP  [Similar Products]
UniProt Comments for CEBPb
Important transcription factor regulating the expression of genes involved in immune and inflammatory responses (PubMed:16585579, PubMed:17911624, PubMed:18486321, PubMed:20111005). Plays also a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis (PubMed:9727068, PubMed:10635333, PubMed:17301242, PubMed:17601773, PubMed:19478079, PubMed:24061474, PubMed:24216764). The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant functions with CEBPA (PubMed:15509779). Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage (PubMed:9727068, PubMed:10635333, PubMed:16585579). Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Plays also a role in intracellular bacteria killing (PubMed:17911624). During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation (PubMed:15985551, PubMed:17301242, PubMed:17601773, PubMed:20194620). Essential for female reproduction because of a critical role in ovarian follicle development (PubMed:9303532). Restricts osteoclastogenesis (PubMed:19440205).
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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