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Embryonic Fibroblasts, P3, Mitomycin-C Treated Cell

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Catalog # MBS652599
Unit / Price
  5 Vials  /  $490 +1 FREE 8GB USB
Embryonic Fibroblasts, P3, Mitomycin-C Treated cell
Product Name

Embryonic Fibroblasts, P3, Mitomycin-C Treated, Cell

Also Known As

Embryonic Fibroblasts, P3, Mitomycin-C Treated, Mouse (CF-1 MEF)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Purity/Purification
Molecular Biology Grade
Note
All cell lines are produced under aseptic conditions. Each lot is tested to be free from bacterial/fungal, mouse pathogen and mycoplasma contamination before shipping.
ANALYSIS PARAMETERS CRITERION RESULT Viability Test1 > 60% 95% Sterility Test2 no growth no growth Mouse Pathogen Test3 negative negative Mycoplasma4 negative negative Mouse ESC support5 pass pass
1. Viability is defined as the ratio of number of live cells over total number of cells after thawing. 2. Immersion method is used to inoculate directly into Tryptic Soy Broth media and Fluid Thioglycollate media to check for bacteria and fungal contamination. 3. PCR based assays for mouse pathogens including Sendai virus, mouse hepatitis virus, pneumonia virus, minute virus, parvovirus (MPV1,MPV2, MPV3) and Theiler's murine encephalomyelitis virus. 4. PCR based assay. 5. Mouse embryonic stem cells are plated and observed for growth on a layer of feeder cells by ES cell colony morphology.
Preparation and Storage
Vapor phase of liquid nitrogen freezer
Other Notes
Small volumes of Embryonic Fibroblasts, P3, Mitomycin-C Treated cell vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
MEF CF1 mitomycin-C treated

Mouse embryonic fibroblast cells. Passage #3, irradiated, ~4x10e6 cells/vial
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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