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CERK elisa kit :: Human ceramide kinase ELISA Kit

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Catalog # MBS919327
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Typical Testing Data/Standard Curve (for reference only)
Product Name

ceramide kinase (CERK), ELISA Kit

Popular Item
Full Product Name

Human Ceramide kinase, CERK ELISA Kit

Product Synonym Names
Human Ceramide kinase (CERK) ELISA kit; RP6-59H18.1; DKFZp434E0211; FLJ21430; FLJ23239; KIAA1646; LK4; MGC131878; dA59H18.2; dA59H18.3; hCERK; lipid kinase LK4; ceramide kinase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS919327 Testing Data
OMIM
610307
3D Structure
ModBase 3D Structure for Q8TCT0
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human CERK. No significant cross-reactivity or interference between human CERK and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
18.75 pg/ml -1200 pg/ml
Sensitivity
4.67 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CERK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CERKpurchase
MBS919327 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ceramide kinase (CERK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CERK. The ELISA analytical biochemical technique of the MBS919327 kit is based on CERK antibody-CERK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CERK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CERK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CERK elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CERK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CERK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CERK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CERK bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CERK elisa kit
CERK elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS919327. Click to request current manual
NCBI/Uniprot data below describe general gene information for CERK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,977 Da
NCBI Official Full Name
ceramide kinase
NCBI Official Synonym Full Names
ceramide kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
LK4; hCERK; dA59H18.2; dA59H18.3
  [Similar Products]
NCBI Protein Information
ceramide kinase; lipid kinase 4; lipid kinase LK4; acylsphingosine kinase
UniProt Protein Name
Ceramide kinase
UniProt Synonym Protein Names
Acylsphingosine kinase; Lipid kinase 4; LK4
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
KIAA1646; hCERK; LK4  [Similar Products]
UniProt Entry Name
CERK1_HUMAN
NCBI Summary for CERK
CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM, Mar 2008]
UniProt Comments for CERK
CERK: Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides.

Protein type: EC 2.7.1.138; Kinase, lipid; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: mitochondrion; plasma membrane; integral to membrane

Molecular Function: ceramide kinase activity; magnesium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: sphingolipid metabolic process; protein kinase C activation; glycosphingolipid metabolic process; ceramide metabolic process; lipid phosphorylation; transmembrane transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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