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CFTR elisa kit :: Porcine Cystic Fibrosis Transmembrane Conductance Regulator ELISA Kit

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Catalog # MBS010824
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
CFTR elisa kit
Product Name

Cystic Fibrosis Transmembrane Conductance Regulator (CFTR), ELISA Kit

Popular Item
Full Product Name

Porcine Cystic Fibrosis Transmembrane Conductance Regulator ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 7; NC_000007.13 (117120017..117308719). Location: 7q31.2
OMIM
219700
3D Structure
ModBase 3D Structure for P13569
Species Reactivity
Specificity
No significant cross-reactivity or interference between Porcine CFTR and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Assay Type
Sandwich
Detection Range
0.625 ng/ml - 20 ng/ml.
Sensitivity
0.1 ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CFTR concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine CFTR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CFTR and CFTR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CFTR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CFTR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCFTRpurchase
MBS010824 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CFTR. The ELISA analytical biochemical technique of the MBS010824 kit is based on CFTR antibody-CFTR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CFTR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CFTR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CFTR elisa kit
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CFTR concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine CFTR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CFTR and CFTR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CFTR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS010824. Click to request current manual
NCBI/Uniprot data below describe general gene information for CFTR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
168,142 Da
NCBI Official Full Name
CFTR, partial
NCBI Official Synonym Full Names
cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
NCBI Official Symbol
NCBI Official Synonym Symbols
CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1
  [Similar Products]
NCBI Protein Information
cystic fibrosis transmembrane conductance regulator; cAMP-dependent chloride channel; channel conductance-controlling ATPase
UniProt Protein Name
Cystic fibrosis transmembrane conductance regulator
UniProt Synonym Protein Names
ATP-binding cassette sub-family C member 7; Channel conductance-controlling ATPase (EC:3.6.3.49); cAMP-dependent chloride channel
UniProt Gene Name
UniProt Synonym Gene Names
ABCC7; CFTR  [Similar Products]
UniProt Entry Name
CFTR_HUMAN
NCBI Summary for CFTR
This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for CFTR
CFTR: a member of the MRP subfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. Functions as a chloride channel and controls the regulation of other transport pathways. Mutations have been observed in patients with the autosomal recessive disorders cystic fibrosis (CF) and congenital bilateral aplasia of the vas deferens (CBAVD). Alternative splice

Protein type: Hydrolase; Membrane protein, integral; Transporter, ABC family; Transporter; Channel, chloride; EC 3.6.3.49; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q31.2

Cellular Component: recycling endosome; protein complex; microvillus; cytoplasmic vesicle membrane; cell surface; early endosome membrane; basolateral plasma membrane; early endosome; apical plasma membrane; cytoplasm; plasma membrane

Molecular Function: bicarbonate transmembrane transporter activity; protein binding; chloride channel activity; enzyme binding; chloride channel inhibitor activity; chloride transmembrane transporter activity; channel-conductance-controlling ATPase activity; ATP-binding and phosphorylation-dependent chloride channel activity; ATP binding; PDZ domain binding

Biological Process: response to drug; intracellular pH elevation; response to peptide hormone stimulus; cholesterol transport; iodide transport; water transport; transepithelial chloride transport; membrane hyperpolarization; respiratory gaseous exchange; positive regulation of vasodilation; cholesterol biosynthetic process; vasodilation; cellular response to hormone stimulus; bicarbonate transport; response to estrogen stimulus; transport; response to cytokine stimulus; transmembrane transport; sperm capacitation; lung development

Disease: Vas Deferens, Congenital Bilateral Aplasia Of; Bronchiectasis With Or Without Elevated Sweat Chloride 1; Cystic Fibrosis; Pancreatitis, Hereditary
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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