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CGRP elisa kit :: Human CGRP ELISA Kit

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Catalog # MBS2500141
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CGRP, ELISA Kit

Popular Item
Also Known As

Human CGRP (Calcitonin Gene Related Peptide) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2500141 COA
MBS2500141 Testing Data
Sequence Length
128
OMIM
114130
3D Structure
ModBase 3D Structure for P06881
Species Reactivity
Specificity
This kit recognizes natural and some recombinant Human CGRP. No significant crossreactivity or interference between Human CGRP and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CGRP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCGRPpurchase
MBS2500141 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CGRP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CGRP. The ELISA analytical biochemical technique of the MBS2500141 kit is based on CGRP antibody-CGRP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CGRP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CGRP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CGRP elisa kit
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human CGRP concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human CGRP. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human CGRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human CGRP, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CGRP. The concentration of Human CGRP in samples can be calculated by comparing the OD of the samples with the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CGRP elisa kit
CGRP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2500141. Click to request current manual
NCBI/Uniprot data below describe general gene information for CGRP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,466 Da
NCBI Official Full Name
calcitonin gene-related peptide 1 isoform alpha-CGRP preproprotein
NCBI Official Synonym Full Names
calcitonin-related polypeptide alpha
NCBI Official Symbol
CALCA  [Similar Products]
NCBI Official Synonym Symbols
CT; KC; CGRP; CALC1; CGRP1; CGRP-I
  [Similar Products]
NCBI Protein Information
calcitonin; calcitonin gene-related peptide 1; alpha-type CGRP; calcitonin 1; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha; katacalcin
UniProt Protein Name
Calcitonin gene-related peptide 1
UniProt Synonym Protein Names
Alpha-type CGRP; Calcitonin gene-related peptide I; CGRP-I
UniProt Gene Name
CALCA  [Similar Products]
UniProt Synonym Gene Names
CALC1; CGRP-I  [Similar Products]
UniProt Entry Name
CALCA_HUMAN
NCBI Summary for CGRP
This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]
UniProt Comments for CGRP
CALCA iso3: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular space; cell soma; cytoplasm; extracellular region; terminal button; nucleus

Molecular Function: identical protein binding; protein binding; calcitonin receptor binding; hormone activity; protein complex binding; receptor binding

Biological Process: negative regulation of osteoclast differentiation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of interleukin-1 alpha production; vasodilation of artery during baroreceptor response to increased systemic arterial blood pressure; response to pain; positive regulation of vasodilation; G-protein signaling, adenylate cyclase activating pathway; protein amino acid phosphorylation; positive regulation of interleukin-8 production; monocyte chemotaxis; elevation of cytosolic calcium ion concentration; leukocyte adhesion; vasculature development; cell-cell signaling; neuropeptide signaling pathway; regulation of blood pressure; negative regulation of smooth muscle contraction; receptor internalization; positive regulation of cAMP biosynthetic process; negative regulation of ossification; feeding behavior; positive regulation of adenylate cyclase activity; negative regulation of blood pressure; inflammatory response; positive regulation of macrophage differentiation; detection of temperature stimulus involved in sensory perception of pain; negative regulation of bone resorption; negative regulation of neurological process; aging; cytosolic calcium ion homeostasis; regulation of heart rate; adenylate cyclase activation; positive regulation of ossification; regulation of systemic arterial blood pressure by neurological process; G-protein coupled receptor internalization; response to heat; endothelial cell proliferation; regulation of inflammatory response; activation of protein kinase activity; endothelial cell migration; negative regulation of transcription, DNA-dependent; embryo implantation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with CGRP elisa kit
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